Differential expression of three sialidase genes in rat development

Takafumi Hasegawa, Carmen Feijoo Carnero, Tadashi Wada, Taeko Miyagi, Takafumi Hasegawa, Yasuto Itoyama

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Mammalian sialidases have been reported to give a great influence on a number of cellular functions including cell differentiation and cell growth by removal of sialic acids from glycoproteins and gangliosides. To understand the roles of the sialidases during development, we investigated expression pattern of three types of sialidase in developing rat brain and liver. For this purpose we cloned a new membrane-associated sialidase cDNA from rat brain. The cDNA encodes 418 amino acids containing three ASP-boxes characteristic of sialidases and the major transcript of 3.5 kb is highly expressed in brain and cardiac muscle but low in liver. Competitive polymerase chain reaction methods were developed to evaluate the mRNA level together with activity assays in comparison with cytosolic and lysosomal sialidases previously obtained. The results indicate that the expression of individual sialidase genes is spatiotemporally controlled with distinct roles in determining the concentration and components of sialo-glycoconjugates during development.

Original languageEnglish
Pages (from-to)726-732
Number of pages7
JournalBiochemical and biophysical research communications
Volume280
Issue number3
DOIs
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • Competitive PCR
  • Development
  • Gangliosides
  • Sialic acid
  • Sialidase
  • cDNA cloning

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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