Differential expression of the nonmuscle-type cofilin gene between subcutaneous and visceral adipose tissue

Ki Choon Choi, Sang Gun Roh, Daisuke Hishikawa, Hisae Miyahara, Masaaki Kuno, Hiroaki Tsuzuki, Ai Tomimatsu, Yeon Hee Hong, Kwang Keun Cho, Kyung Ho Han, Shin Ichi Sasaki

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Visceral adipocytes differ in various biochemical properties from adipocytes of subcutaneous origin. However, information on differences in gene expression between visceral and subcutaneous fat depots is limited. Expression of the genes for the nonmuscle and muscle isoforms of the actin-binding protein cofilin was examined in subcutaneous and visceral fat depots of mice, pigs, and cattle by semiquantative reverse transcription and polymerase chain reaction analysis. The abundance of nonmuscle-type cofilin mRNA was markedly higher in visceral adipose tissue than in subcutaneous adipose tissue of mouse and pig. This difference was more pronounced in mice fed a high-fat diet than in those fed a standard diet. In cattle, however, the amount of non-muscle-type cofilin mRNA was greater in subcutaneous fat than in visceral fat. Muscle-type cofilin mRNA was not detected in either adipose tissue of any of the three species. These results suggest that the nonmuscle isoform of cofilin, and therefore the cytoskeleton, may play a role in lipid accumulation in visceral adipose tissue.

Original languageEnglish
Pages (from-to)2262-2265
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume67
Issue number10
DOIs
Publication statusPublished - 2003 Oct
Externally publishedYes

Keywords

  • Nonmuscle-type cofilin gene
  • Subcutaneous adipocyte
  • Visceral adipocyte

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Differential expression of the nonmuscle-type cofilin gene between subcutaneous and visceral adipose tissue'. Together they form a unique fingerprint.

Cite this