Differential expression of human beta-defensin 2 in keratinized and non-keratinized oral epithelial lesions; immunohistochemistry and in situ hybridization

Yoshihiro Abiko, Anura K. Suraweera, Michiko Nishimura, Toshiya Arakawa, Taishin Takuma, Itaru Mizoguchi, Tohru Kaku

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

Human β-defensin(hBD)-2, an antimicrobial peptide, is produced by various epithelial cells. Because hBD-2 expression in the oral epithelium has not been assessed, we investigated its localization in normal oral epithelium and epithelial lesions. hBD-2 expression was studied using immunohistochemistry and in situ hybridization on formalin-fixed, paraffin-embedded tissue sections from 30 cases of squamous cell carcinoma and 6 cases of leukoplakia. Immunostaining for hBD-2 was more intense in hyperkeratinized than in ortho- or non-keratinized epithelium. In contrast, signals for hBD-2 mRNA were frequently stronger in non-keratinized epithelium than in hyper- or ortho-keratinized epithelium. The results suggest that keratinization in oral epithelium plays an important role in the biological function of hBD-2 both at the mRNA level and in the retention of the peptide in the epithelium.

Original languageEnglish
Pages (from-to)248-253
Number of pages6
JournalVirchows Archiv
Volume438
Issue number3
DOIs
Publication statusPublished - 2001 Apr 10
Externally publishedYes

Keywords

  • Antimicrobial peptide
  • Defensin
  • Oral
  • RT-PCR
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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