TY - JOUR
T1 - Differential expression of AtXTH17, AtXTH18, AtXTH19 and AtXTH20 genes in Arabidopsis roots. Physiological roles in specification in cell wall construction
AU - Vissenberg, Kris
AU - Oyama, Mika
AU - Osato, Yasue
AU - Yokoyama, Ryusuke
AU - Verbelen, Jean Pierre
AU - Nishitani, Kazuhiko
N1 - Funding Information:
The authors greatly acknowledge the gift of XGO-SRs by Professor S.C. Fry (University of Edinburgh, U.K.). This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas (15031202) and Scientific Research (B) (15370016) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and by the Program for ‘Development of Fundamental Technologies for Controlling the Process of Material Production of Plants’ from the New Energy and Industrial Technology Development Organization, Japan. K.V. is a post-doctoral fellow of the Fund for Scientific Research Flanders (FWO-Belgium).
PY - 2005/1
Y1 - 2005/1
N2 - Xyloglucan endotransglucosylase/hydrolases (XTHs) are a class of enzymes that are capable of splitting and reconnecting xyloglucan molecules, and are implicated in the construction and restructuring of the cellulose/xyloglucan framework. Thirty-three members of the XTH gene family are found in the genome of Arabidopsis thaliana, but their roles remain unclear. Here, we describe the tissue-specific and growth stage-dependent expression profiles of promoter::GUS fusion constructs for four Arabidopsis XTH genes, AtXTH17, AtXTH18, AtXTH19 and AtXTH20, which are phylogenetically closely related to one another. AtXTH17 and AtXTH18 were expressed in all cell types in the elongating and differentiating region of the root, while AtXTH19 was expressed in the apical dividing and elongating regions, as well as in the differentiation zone, and was up-regulated by auxin. In contrast, AtXTH20 was expressed specifically in vascular tissues in the basal mature region of the root. This expression analysis also disclosed cis-regulatory sequences that are conserved among the four genes, and are responsible for the root-specific expression profile. These results indicate that the four XTH genes, which were generated by gene duplication, have diversified their expression profile within the root in such a way as to take responsibility for particular physiological roles in the cell wall dynamics. JSPP
AB - Xyloglucan endotransglucosylase/hydrolases (XTHs) are a class of enzymes that are capable of splitting and reconnecting xyloglucan molecules, and are implicated in the construction and restructuring of the cellulose/xyloglucan framework. Thirty-three members of the XTH gene family are found in the genome of Arabidopsis thaliana, but their roles remain unclear. Here, we describe the tissue-specific and growth stage-dependent expression profiles of promoter::GUS fusion constructs for four Arabidopsis XTH genes, AtXTH17, AtXTH18, AtXTH19 and AtXTH20, which are phylogenetically closely related to one another. AtXTH17 and AtXTH18 were expressed in all cell types in the elongating and differentiating region of the root, while AtXTH19 was expressed in the apical dividing and elongating regions, as well as in the differentiation zone, and was up-regulated by auxin. In contrast, AtXTH20 was expressed specifically in vascular tissues in the basal mature region of the root. This expression analysis also disclosed cis-regulatory sequences that are conserved among the four genes, and are responsible for the root-specific expression profile. These results indicate that the four XTH genes, which were generated by gene duplication, have diversified their expression profile within the root in such a way as to take responsibility for particular physiological roles in the cell wall dynamics. JSPP
KW - Arabidopsis
KW - Auxin
KW - Cell wall
KW - Promoter
KW - Root
KW - XTH
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U2 - 10.1093/pcp/pci013
DO - 10.1093/pcp/pci013
M3 - Article
C2 - 15659443
AN - SCOPUS:14644401119
VL - 46
SP - 192
EP - 200
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 1
ER -