Differential expression and regulatory roles of FABP5 and FABP7 in oligodendrocyte lineage cells

Kazem Sharifi, Majid Ebrahimi, Yoshiteru Kagawa, Ariful Islam, Tuerhong Tuerxun, Yuki Yasumoto, Tomonori Hara, Yui Yamamoto, Hirofumi Miyazaki, Nobuko Tokuda, Takeo Yoshikawa, Yuji Owada

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Fatty-acid-binding proteins (FABPs) are key intracellular molecules involved in the uptake, transportation and storage of fatty acids and in the mediation of signal transduction and gene transcription. However, little is known regarding their expression and function in the oligodendrocyte lineage. We evaluate the in vivo and in vitro expression of FABP5 and FABP7 in oligodendrocyte lineage cells in the cortex and corpus callosum of adult mice, mixed cortical culture and oligosphere culture by immunofluorescent counter-staining with major oligodendrocyte lineage markers. In all settings, FABP7 expression was detected in NG2+/PDGFRα+ oligodendrocyte progenitor cells (OPCs) that did not express FABP5. FABP5 was detected in mature CC1+/MBP+ oligodendrocytes that did not express FABP7. Analysis of cultured OPCs showed a significant decrease in the population of FABP7-knockout (KO) OPCs and their BrdU uptake compared with wild-type (WT) OPCs. Upon incubation of OPCs in oligodendrocyte differentiation medium, a significantly lower percentage of FABP7-KO OPCs differentiated into O4+ oligodendrocytes. The percentage of mature MBP+ oligodendrocytes relative to whole O4+/MBP+ oligodendrocytes was significantly lower in FABP7-KO and FABP5-KO than in WT cell populations. The percentage of terminally mature oligodendrocytes with membrane sheet morphology was significantly lower in FABP5-KO compared with WT cell populations. Thus, FABP7 and FABP5 are differentially expressed in oligodendrocyte lineage cells and regulate their proliferation and/or differentiation. Our findings suggest the involvement of FABP7 and FABP5 in the pathophysiology of demyelinating disorders, neuropsychiatric disorder and glioma, conditions in which OPCs/oligodendrocytes play central roles.

Original languageEnglish
Pages (from-to)683-695
Number of pages13
JournalCell and Tissue Research
Volume354
Issue number3
DOIs
Publication statusPublished - 2013 Dec 1

Keywords

  • Differentiation
  • FABP
  • Oligodendrocyte
  • Oligodendrocyte progenitor cells
  • Proliferation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Fingerprint Dive into the research topics of 'Differential expression and regulatory roles of FABP5 and FABP7 in oligodendrocyte lineage cells'. Together they form a unique fingerprint.

  • Cite this

    Sharifi, K., Ebrahimi, M., Kagawa, Y., Islam, A., Tuerxun, T., Yasumoto, Y., Hara, T., Yamamoto, Y., Miyazaki, H., Tokuda, N., Yoshikawa, T., & Owada, Y. (2013). Differential expression and regulatory roles of FABP5 and FABP7 in oligodendrocyte lineage cells. Cell and Tissue Research, 354(3), 683-695. https://doi.org/10.1007/s00441-013-1730-7