Differential effects of forced swim-stress on the corticotropin-releasing hormone and vasopressin gene transcription in the parvocellular division of the paraventricular nucleus of rat hypothalamus

Ya Qiu Jiang, Hisayoshi Kawashima, Yasumasa Iwasaki, Katsuya Uchida, Koreaki Sugimoto, Keiichi Itoi

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Corticotropin-releasing hormone (CRH) and vasopressin (AVP) colocalize in the parvocellular division of the paraventricular nucleus of the hypothalamus (PVN). We examined the effect of forced swim-stress on the CRH and AVP primary transcript (hnRNA) levels in the rat PVN by semi-quantitative in situ hybridization. CRH hnRNA increased markedly following 10-min swim-stress and returned to the basal level by 2 h. AVP hnRNA in the parvocellular division of the PVN, where AVP colocalizes with CRH, did not change significantly immediately after the swim-stress, but it did increase significantly 2 h after the stress. Pretreatment with dexamethasone abolished the increases in CRH and AVP hnRNA levels after the swim-stress. The present results demonstrate the differential effects of forced swim-stress on the CRH and AVP gene transcription in the parvocellular PVN, confirming the diverse response of the dual peptide-containing system in the face of acute stressful events.

Original languageEnglish
Pages (from-to)201-204
Number of pages4
JournalNeuroscience Letters
Volume358
Issue number3
DOIs
Publication statusPublished - 2004 Apr 1

Keywords

  • Adrenocorticotropin
  • Exon
  • Gene
  • In situ hybridization
  • Intron
  • RNA

ASJC Scopus subject areas

  • Neuroscience(all)

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