Differential effects of clarithromycin and azithromycin on delayed rectifier K+-channel currents in murine thymocytes

Itsuro Kazama, Yoshio Maruyama

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Context: Lymphocytes predominantly express delayed rectifier K +-channels (Kv1.3) in their plasma membranes, and the channels play crucial roles in the lymphocyte activation and proliferation. Since macrolide antibiotics, such as clarithromycin and azithromycin, exert immunomodulatory effects, they would affect the Kv1.3-channel currents in lymphocytes. Objective: This study determined the physiological involvement in the mechanisms of immunomodulation by these antibiotics. Materials and methods: Employing the standard patch-clamp whole-cell recording technique in murine thymocytes, we examined the effects of 30 and 100M clarithromycin and azithromycin on the Kv1.3-channel currents and the membrane capacitance. Results: Clarithromycin significantly suppressed the peak currents (30M, 178±5.6 to 111±2.0pA/pF; 100M, 277±4.4 to 89.6±10pA/pF) and the pulse-end currents (30M, 47.5±2.2% to 15.5±3.3%; 100M, 48.5±1.4% to 15.8±1.0%) of thymocyte Kv1.3-channels without significant effects on the membrane capacitance. In contrast, azithromycin did not affect the channel currents. However, it significantly decreased the membrane capacitance (30M, 4.68±0.14 to 3.74±0.13pF; 100M, 4.47±0.06 to 3.37±0.08pF), indicating its accumulation in the plasma membrane. Discussion and conclusion: This study demonstrated for the first time that clarithromycin exerts inhibitory effects on thymocyte Kv1.3-channel currents, while azithromycin decreases the membrane capacitance without affecting the channel currents. These differences in the effects of the macrolide antibiotics may reflect differences in the mechanisms of immunomodulation by which they control the production of cytokines.

Original languageEnglish
Pages (from-to)760-765
Number of pages6
JournalPharmaceutical Biology
Volume51
Issue number6
DOIs
Publication statusPublished - 2013 Jun 1

Keywords

  • Immunomodulatory effects
  • Kv1.3-channel
  • Lymphocytes
  • Macrolides
  • Membrane capacitance

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

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