Differential effect of epidermal growth factor on serous and mucous cells in porcine airway submucosal gland

Nobuhisa Iwase, Tsukasa Sasaki, Takako Oshiro, Tsutomu Tamada, Masayuki Nara, Kan Sasamori, Toshio Hattori, Kunio Shirato, Yoshio Maruyama

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Using a patch-clamp technique, we found that the fresh porcine submucosal gland acinar cells contained two functionally distinct cell populations, i.e. physiologically relevant concentration of acetylcholine (ACh, 30 nM) induced two distinct patterns of electric response in tracheal gland acinar cells. One was characterized by an outstanding oscillatory Cl--current activity, and the other was with poor Cl--current response but with a comparable K+-current. We examined the effect of epidermal growth factor (EGF) on the ACh-induced electric responses in these cells. EGF affected only the latter (K+-prominent) cell type to potentiate significantly the ACh-induced K+-current. An immunohistochemistry revealed that the receptor for EGF was identified preferentially on the mucous, but not serous, cells. Genistein, one of the tyrosine-kinase inhibitors, abolished the augmentation effect of EGF on the ACh-induced current. Thus, we identified the serous cell with a Cl--rich current in response to ACh and the mucous cell with a K+-dominant response. Moreover, EGF affected the mucous cells alone to potentiate the ACh-induced electric response. EGF may contribute to the pathophysiological alterations in chronic inflammatory airways both in morphological (mucous cell hypertrophy/hyperplasia) and functional (thick viscous hypersecretion) ways.

Original languageEnglish
Pages (from-to)307-319
Number of pages13
JournalRespiratory Physiology and Neurobiology
Volume132
Issue number3
DOIs
Publication statusPublished - 2002 Sep 4

Keywords

  • Currents, Cl, K, trachea submucosal gland cells
  • Gland, submucosal
  • Mammals, pig
  • Mediators, EGF
  • Pharmacological agents, genistein
  • Receptor, EGF
  • Trachea, gland acinar cells

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology
  • Pulmonary and Respiratory Medicine

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