TY - JOUR
T1 - Differential distribution of TRP Ca2+ channel isoforms in mouse brain
AU - Mori, Yasuo
AU - Takada, Naoyuki
AU - Okada, Takaharu
AU - Wakamori, Minoru
AU - Imoto, Keiji
AU - Wanifuchi, Hiroshi
AU - Oka, Hiroaki
AU - Oba, Akio
AU - Ikenaka, Kazuhiro
AU - Kurosaki, Tomohiro
PY - 1998/2/16
Y1 - 1998/2/16
N2 - MAMMALIAN homologues of the Drosophila TRP proteins, which are essential for light-activated, phosphatidyl-inositide (PI)-dependent Ca2+ conductance in Drosophila photoreceptors, were molecularly identified, to investigate receptor-activated Ca2+ influx in the mammalian nervous system. Two cloned mouse TRP homologues, TRP3 and TRP4, structurally related to the voltage- dependent Na+ channel, were expressed predominantly in the brain, where a sharp contrast in the distribution of the RNA transcripts for TRP isoforms was demonstrated by in situ hybridization analysis. TRP3 mRNA was concentrated in cerebellar Purkinje cells and sparsely localized in the cerebellar granule layer, pontine nuclei, and thalamus, whereas TRP4 mRNA was abundantly expressed in hippocampal CA1 pyramidal neurons, dentate gyrus granule cells, and cerebral cortical neurons, and in the septal nuclei and the mitral layer of olfactory bulb. The distinct special patterns of TRP isoforms implicate that neurons are highly heterogeneous in receptor- activated Ca2+ influx responsible for the second phase of PI-mediated rise in intracellular Ca2+.
AB - MAMMALIAN homologues of the Drosophila TRP proteins, which are essential for light-activated, phosphatidyl-inositide (PI)-dependent Ca2+ conductance in Drosophila photoreceptors, were molecularly identified, to investigate receptor-activated Ca2+ influx in the mammalian nervous system. Two cloned mouse TRP homologues, TRP3 and TRP4, structurally related to the voltage- dependent Na+ channel, were expressed predominantly in the brain, where a sharp contrast in the distribution of the RNA transcripts for TRP isoforms was demonstrated by in situ hybridization analysis. TRP3 mRNA was concentrated in cerebellar Purkinje cells and sparsely localized in the cerebellar granule layer, pontine nuclei, and thalamus, whereas TRP4 mRNA was abundantly expressed in hippocampal CA1 pyramidal neurons, dentate gyrus granule cells, and cerebral cortical neurons, and in the septal nuclei and the mitral layer of olfactory bulb. The distinct special patterns of TRP isoforms implicate that neurons are highly heterogeneous in receptor- activated Ca2+ influx responsible for the second phase of PI-mediated rise in intracellular Ca2+.
KW - Capacitative Ca entry
KW - IP
KW - Receptor-activated Ca channel
KW - Stored-operated channel
KW - TRP
KW - Tissue distribution
KW - cDNA cloning
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M3 - Article
C2 - 9512398
AN - SCOPUS:15144355753
VL - 9
SP - 507
EP - 515
JO - NeuroReport
JF - NeuroReport
SN - 0959-4965
IS - 3
ER -