Differential distribution of TRP Ca2+ channel isoforms in mouse brain

Yasuo Mori, Naoyuki Takada, Takaharu Okada, Minoru Wakamori, Keiji Imoto, Hiroshi Wanifuchi, Hiroaki Oka, Akio Oba, Kazuhiro Ikenaka, Tomohiro Kurosaki

Research output: Contribution to journalArticlepeer-review

65 Citations (Scopus)

Abstract

MAMMALIAN homologues of the Drosophila TRP proteins, which are essential for light-activated, phosphatidyl-inositide (PI)-dependent Ca2+ conductance in Drosophila photoreceptors, were molecularly identified, to investigate receptor-activated Ca2+ influx in the mammalian nervous system. Two cloned mouse TRP homologues, TRP3 and TRP4, structurally related to the voltage- dependent Na+ channel, were expressed predominantly in the brain, where a sharp contrast in the distribution of the RNA transcripts for TRP isoforms was demonstrated by in situ hybridization analysis. TRP3 mRNA was concentrated in cerebellar Purkinje cells and sparsely localized in the cerebellar granule layer, pontine nuclei, and thalamus, whereas TRP4 mRNA was abundantly expressed in hippocampal CA1 pyramidal neurons, dentate gyrus granule cells, and cerebral cortical neurons, and in the septal nuclei and the mitral layer of olfactory bulb. The distinct special patterns of TRP isoforms implicate that neurons are highly heterogeneous in receptor- activated Ca2+ influx responsible for the second phase of PI-mediated rise in intracellular Ca2+.

Original languageEnglish
Pages (from-to)507-515
Number of pages9
JournalNeuroReport
Volume9
Issue number3
Publication statusPublished - 1998 Feb 16
Externally publishedYes

Keywords

  • Capacitative Ca entry
  • IP
  • Receptor-activated Ca channel
  • Stored-operated channel
  • TRP
  • Tissue distribution
  • cDNA cloning

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint

Dive into the research topics of 'Differential distribution of TRP Ca<sup>2+</sup> channel isoforms in mouse brain'. Together they form a unique fingerprint.

Cite this