TY - JOUR
T1 - Differential cytokine gene expression in CD4+ and CD8+ T cell subsets of calves
AU - Tanaka, Sachi
AU - Aso, Hisashi
AU - Miyazawa, Kohtaro
AU - Nagai, Yasuhiro
AU - Watanabe, Kouichi
AU - Ohwada, Shyuichi
AU - Kobayashi, Jin
AU - Yamaguchi, Takahiro
N1 - Funding Information:
This study was supported by Development of Stable Production Technology of Clone Animals by Somatic Cell Nuclear Transfer (Development of Biotechnology Safety).
PY - 2007/7/15
Y1 - 2007/7/15
N2 - The distinct patterns of cytokine expression in CD4+ and CD8+ T cells are well understood in mice and humans. However, little information is available about cytokine expression in bovine CD4+ and CD8+ T cells. In this study, mRNA expression of 19 different cytokines was analyzed in CD4+ and CD8+ T cells of calves with or without Concanavalin A (Con A) stimulation. CD4+ and CD8+ T cell populations were enriched to 98% purity by positive selection using magnetic cell sorting (MACS). CD4+ T cells spontaneously expressed the mRNAs of interleukin-1α (IL-1α), IL-1β, IL-2, IL-6, IL-7, IL-8, IL-10, IL-18, IFN-γ, TNF-α, TNF-β and TGF-β, and augmented the mRNA expression of IL-10, IFN-γ and TNF-β after Con A stimulation. The mRNAs of IL-3, IL-4, IL-5, IL-13 and GM-CSF were newly expressed in Con A-stimulated CD4+ T cells. CD8+ T cells displayed spontaneous mRNA expression of IL-6, IL-18, TNF-α, TNF-β and TGF-β, and newly expressed the mRNA of IL-2, IL-7, interferon-gamma (IFN-γ) and GM-CSF after Con A stimulation. It was found that CD4+ T cells expressed the mRNA of 17 cytokines except for IL-12 and IL-15, while CD8+ T cells expressed only the mRNA of 9 cytokines after Con A stimulation. The profile of cytokine mRNA expression was substantially different in the CD4+ and CD8+ T cells of calves, indicating that CD4+ T cells can be distinguished from CD8+ T cells by the cytokine gene expression of IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-8, IL-10 and IL-13. Differential cytokine expression between CD4+ and CD8+ T cells serve to interpret an individual function of T cell subsets in the immune system of calves.
AB - The distinct patterns of cytokine expression in CD4+ and CD8+ T cells are well understood in mice and humans. However, little information is available about cytokine expression in bovine CD4+ and CD8+ T cells. In this study, mRNA expression of 19 different cytokines was analyzed in CD4+ and CD8+ T cells of calves with or without Concanavalin A (Con A) stimulation. CD4+ and CD8+ T cell populations were enriched to 98% purity by positive selection using magnetic cell sorting (MACS). CD4+ T cells spontaneously expressed the mRNAs of interleukin-1α (IL-1α), IL-1β, IL-2, IL-6, IL-7, IL-8, IL-10, IL-18, IFN-γ, TNF-α, TNF-β and TGF-β, and augmented the mRNA expression of IL-10, IFN-γ and TNF-β after Con A stimulation. The mRNAs of IL-3, IL-4, IL-5, IL-13 and GM-CSF were newly expressed in Con A-stimulated CD4+ T cells. CD8+ T cells displayed spontaneous mRNA expression of IL-6, IL-18, TNF-α, TNF-β and TGF-β, and newly expressed the mRNA of IL-2, IL-7, interferon-gamma (IFN-γ) and GM-CSF after Con A stimulation. It was found that CD4+ T cells expressed the mRNA of 17 cytokines except for IL-12 and IL-15, while CD8+ T cells expressed only the mRNA of 9 cytokines after Con A stimulation. The profile of cytokine mRNA expression was substantially different in the CD4+ and CD8+ T cells of calves, indicating that CD4+ T cells can be distinguished from CD8+ T cells by the cytokine gene expression of IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-8, IL-10 and IL-13. Differential cytokine expression between CD4+ and CD8+ T cells serve to interpret an individual function of T cell subsets in the immune system of calves.
KW - CD4 T cells
KW - CD8 T cells
KW - Calf
KW - Con A stimulation
KW - Cytokine gene expression
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U2 - 10.1016/j.vetimm.2007.04.013
DO - 10.1016/j.vetimm.2007.04.013
M3 - Article
C2 - 17543393
AN - SCOPUS:34250773318
VL - 118
SP - 84
EP - 91
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
SN - 0165-2427
IS - 1-2
ER -