Differential cytokine expression in T cell subsets from bovine peripheral blood

Sachi Tanaka, Yasuhiro Nagai, Yoko Noguchi, Masato Miyake, Kouichi Watanabe, Shyuichi Ohwada, Hisashi Aso, Takahiro Yamaguchi

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Although distinct cytokine expression in T cell subsets is well understood in mice and humans, limited information is available on bovine T cell subsets. In the present study, we analyzed the mRNA expression of 10 kinds of cytokines and CD25 expression in CD4+, CD8+, WC1+ and WC1- γδ T cell subsets in bovine peripheral blood by Concanavalin A (Con A) stimulation. CD25 expression was significantly increased in CD4+, CD8+ and WC1+ γδ T cells, but not in WC1- γδ T cells by Con A stimulation. In CD4+ T cells, the mRNAs of Interleukin (IL)-2, IL-6, IL-10, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, TNF-β and transforming growth factor (TGF)-β were expressed in control cultures, and IL-3, IL-4 and granulocyte-macrophage colony stimulating factor (GM-CSF) were newly expressed when the cells were stimulated with Con A. CD8+ T cells expressed the mRNAs of IL-6, TNF-α, TNF-β and TGF-β in control cultures, and newly expressed those of IL-2, IFN-γ and GM-CSF, but did not express those of IL-3, IL-4 or IL-10 after Con A stimulation. The cytokine expression profile of WC1+ γδ T cells was similar to that of CD8+ T cells. However, WC1- γδ T cells did not express any cytokine mRNA except TGF-â mRNA. These results will contribute to elucidate the participation of T cell subsets in immune responses against infectious disease in cattle.

Original languageEnglish
Pages (from-to)597-604
Number of pages8
JournalAnimal Science Journal
Volume79
Issue number5
DOIs
Publication statusPublished - 2008 Oct 1

Keywords

  • Cattle
  • Cytokine
  • Peripheral blood
  • T cell subset

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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