TY - JOUR
T1 - Differential cytokine expression in T cell subsets from bovine peripheral blood
AU - Tanaka, Sachi
AU - Nagai, Yasuhiro
AU - Noguchi, Yoko
AU - Miyake, Masato
AU - Watanabe, Kouichi
AU - Ohwada, Shyuichi
AU - Aso, Hisashi
AU - Yamaguchi, Takahiro
PY - 2008/10/1
Y1 - 2008/10/1
N2 - Although distinct cytokine expression in T cell subsets is well understood in mice and humans, limited information is available on bovine T cell subsets. In the present study, we analyzed the mRNA expression of 10 kinds of cytokines and CD25 expression in CD4+, CD8+, WC1+ and WC1- γδ T cell subsets in bovine peripheral blood by Concanavalin A (Con A) stimulation. CD25 expression was significantly increased in CD4+, CD8+ and WC1+ γδ T cells, but not in WC1- γδ T cells by Con A stimulation. In CD4+ T cells, the mRNAs of Interleukin (IL)-2, IL-6, IL-10, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, TNF-β and transforming growth factor (TGF)-β were expressed in control cultures, and IL-3, IL-4 and granulocyte-macrophage colony stimulating factor (GM-CSF) were newly expressed when the cells were stimulated with Con A. CD8+ T cells expressed the mRNAs of IL-6, TNF-α, TNF-β and TGF-β in control cultures, and newly expressed those of IL-2, IFN-γ and GM-CSF, but did not express those of IL-3, IL-4 or IL-10 after Con A stimulation. The cytokine expression profile of WC1+ γδ T cells was similar to that of CD8+ T cells. However, WC1- γδ T cells did not express any cytokine mRNA except TGF-â mRNA. These results will contribute to elucidate the participation of T cell subsets in immune responses against infectious disease in cattle.
AB - Although distinct cytokine expression in T cell subsets is well understood in mice and humans, limited information is available on bovine T cell subsets. In the present study, we analyzed the mRNA expression of 10 kinds of cytokines and CD25 expression in CD4+, CD8+, WC1+ and WC1- γδ T cell subsets in bovine peripheral blood by Concanavalin A (Con A) stimulation. CD25 expression was significantly increased in CD4+, CD8+ and WC1+ γδ T cells, but not in WC1- γδ T cells by Con A stimulation. In CD4+ T cells, the mRNAs of Interleukin (IL)-2, IL-6, IL-10, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, TNF-β and transforming growth factor (TGF)-β were expressed in control cultures, and IL-3, IL-4 and granulocyte-macrophage colony stimulating factor (GM-CSF) were newly expressed when the cells were stimulated with Con A. CD8+ T cells expressed the mRNAs of IL-6, TNF-α, TNF-β and TGF-β in control cultures, and newly expressed those of IL-2, IFN-γ and GM-CSF, but did not express those of IL-3, IL-4 or IL-10 after Con A stimulation. The cytokine expression profile of WC1+ γδ T cells was similar to that of CD8+ T cells. However, WC1- γδ T cells did not express any cytokine mRNA except TGF-â mRNA. These results will contribute to elucidate the participation of T cell subsets in immune responses against infectious disease in cattle.
KW - Cattle
KW - Cytokine
KW - Peripheral blood
KW - T cell subset
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U2 - 10.1111/j.1740-0929.2008.00569.x
DO - 10.1111/j.1740-0929.2008.00569.x
M3 - Article
AN - SCOPUS:50249140681
VL - 79
SP - 597
EP - 604
JO - Animal Science Journal
JF - Animal Science Journal
SN - 1344-3941
IS - 5
ER -