Differential action of trans-10, cis-12 conjugated linoleic acid on adipocyte differentiation of ovine and 3T3-L1 preadipocytes

T. Iga, T. Satoh, S. Yamamoto, K. Fukui, S. H. Song, K. C. Choi, S. G. Roh, S. Sasaki

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Trans-10, cis-12 conjugated linoleic acid (CLA) has been reported to inhibit the adipocyte differentiation of preadipocytes in non-ruminant animals (mice, rat, and human). However, the effects of trans-10, cis-12 CLA have not been clear in ruminants. The objective of this study was to investigate the effects of trans-10, cis-12 CLA on adipocyte differentiation of ovine preadipocytes. Differentiation of these preadipocytes was facilitated by treatment with trans-10, cis-12 CLA. Trans-10, cis-12 CLA increased the number and size of oil red O-stainable lipid drops as well as the levels of GPDH activity. PPAR-γ2 and adipophilin mRNA, adipogenic marker genes, were increased by treatment with trans-10, cis-12 CLA. This result was different from that observed with 3T3-L1 preadipocytes, a clonal cell line derived from rodents. Furthermore, trans-10, cis-12 CLA alone induced the adipocyte differentiation of ovine preadipocytes in differentiation-induction medium without troglitazone. These results suggest that CLA is an inducer and regulator in adipocyte differentiation of ovine preadipocytes, with species differences between ovine and rodent preadipocytes.

Original languageEnglish
Pages (from-to)1566-1573
Number of pages8
JournalAsian-Australasian Journal of Animal Sciences
Volume22
Issue number11
DOIs
Publication statusPublished - 2009 Nov

Keywords

  • Adipocyte
  • Cis-12 CLA
  • Sheep
  • Trans-10

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Engineering(all)

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