TY - JOUR
T1 - Different sensitivities to agonist of muscarinic acetylcholine receptor subtypes
AU - Bujo, Hideaki
AU - Nakai, Junichi
AU - Kubo, Tai
AU - Fukuda, Kazuhiko
AU - Akiba, Isamu
AU - Maeda, Akito
AU - Mishina, Masayoshi
AU - Numa, Shosaku
N1 - Funding Information:
Acknowledgements: We thank Dr KatsunosukeM achida for computera nalysis.T his investigationw as supportedi n part by researchg rants from the Ministry of Education, Sciencea nd Culture of Japan, the Institute of Physical and Chemical Researcht, he MitsubishiF oundationa nd the JapaneseF ounda-tion of Metabolisma nd Diseases.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1988/11/21
Y1 - 1988/11/21
N2 - Muscarinic acetylcholine receptor (mAChR) III expressed in Xenopus oocytes, like mAChR I, mediates activation of a Ca2+-dependent Cl- current, whereas mAChR IV, like mAChR II, principally induces activation of Na+ and K+ currents in a Ca2+-independent manner. mAChR III has a sensitivity to agonist of about one order of magnitude higher than that of mAChR I in mediating the Ca2+-dependent current response in Xenopus oocytes and in stimulating phosphoinositide hydrolysis in NG108-15 neuroblastoma-glioma hybrid cells. The agonist-binding affinity of mAChR III is also about one order of magnitude higher than that of mAChR I.
AB - Muscarinic acetylcholine receptor (mAChR) III expressed in Xenopus oocytes, like mAChR I, mediates activation of a Ca2+-dependent Cl- current, whereas mAChR IV, like mAChR II, principally induces activation of Na+ and K+ currents in a Ca2+-independent manner. mAChR III has a sensitivity to agonist of about one order of magnitude higher than that of mAChR I in mediating the Ca2+-dependent current response in Xenopus oocytes and in stimulating phosphoinositide hydrolysis in NG108-15 neuroblastoma-glioma hybrid cells. The agonist-binding affinity of mAChR III is also about one order of magnitude higher than that of mAChR I.
KW - Agonist-binding affinity
KW - Ca-dependent Cl current
KW - K current
KW - Muscarinic acetylcholine receptor subtype
KW - Na current
KW - Phosphoinositide hydrolysis
KW - Sensitivity to agonist
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U2 - 10.1016/0014-5793(88)80346-6
DO - 10.1016/0014-5793(88)80346-6
M3 - Article
C2 - 3142796
AN - SCOPUS:0024212696
VL - 240
SP - 95
EP - 100
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-2
ER -