Differences in transactivation between rat CYP3A1 and human CYP3A4 genes by human pregnane X receptor.

Tomonari Takada, Makoto Ogino, Masaaki Miyata, Miki Shimada, Kiyoshi Nagata, Yasushi Yamazoe

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)

    Abstract

    In an assay system using a human CYP3A4 reporter constructed with the promoter (+11 nt to -362 nt) and enhancer (-7.2 knt to -7.8 knt) regions including everted repeat separated by six nucleotides (ER-6) and direct repeat separated by three nucleotides (DR-3) motifs, the CYP3A4 transactivation was detected without overexpression of any nuclear receptors in rifampicin-treated HepG2 cells. Overexpression of human pregnane X receptor (hPXR) enhanced the transactivation. Rat CYP3A1 reporter constructed with the promoter region (+31 nt to -171 nt) including both DR-3 and ER-6 motifs was, however, not transactivated in rifampicin-treated cells, even after overexpression of hPXR. Although overexpression of retinoid X receptor alpha (RXRalpha) had no clear effect for both CYP3A reporters, co-expression of apolipoprotein AI regulatory protein-1 (ARP-1) with hPXR resulted in the rifampicin-induced transactivation of the CYP3A1 reporter. A truncated CYP3A4 reporter retaining the both motifs showed the rifampicin-induced transactivation by overexpression of hPXR and ARP-1, while the transactivation in hPXR-overexpressed cells was not observed. These results support the idea that a nuclear receptor other than RXRalpha may play a role in the CYP3A transactivation together with hPXR. The present study also suggests the involvement of a novel cis-element in the hPXR-mediated CYP3A4 transactivation.

    Original languageEnglish
    Pages (from-to)103-113
    Number of pages11
    JournalDrug metabolism and pharmacokinetics
    Volume19
    Issue number2
    DOIs
    Publication statusPublished - 2004 Apr

    ASJC Scopus subject areas

    • Pharmacology
    • Pharmaceutical Science
    • Pharmacology (medical)

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