TY - JOUR
T1 - Differences in the interleukin‐2 (IL‐2) receptor system in human and mouse
T2 - α chain is required for formation of the functional mouse IL‐2 receptor
AU - Nemoto, Tadayoshi
AU - Takeshita, Toshikazu
AU - Ishii, Naoto
AU - Kondo, Motonari
AU - Higuchi, Masaya
AU - Satomi, Susumu
AU - Nakamura, Masataka
AU - Mori, Shozo
AU - Sugamura, Kazuo
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1995/11
Y1 - 1995/11
N2 - Reconstitution with mouse interleukin‐2 (IL‐2) receptor subunits demonstrated that the mouse IL‐2 receptor complex was different from the human complex in the α chain requirement for the functional mouse receptor complex. The heterotrimeric complex of the mouse exogenous α and β chains and the endogenous γ chain on mouse lymphoid BW5147 cells showed the ability to bind IL‐2 with high affinity, resulting in IL‐2‐induced tyrosine phosphorylation of a cytosolic tyrosine kinase, JAK3, which is involved in IL‐2‐dependent signals. Exogenous introduction of the β chain with the endogenous γ chain, however, could neither confer appreciable IL‐2 binding nor IL‐2‐induced signal transduction on BW5147 cells, unlike the human βγ heterodimer. Mouse spleen CD8+ cells, not having the α chain initially, showed IL‐2‐dependent cell proliferation only when expression of the α chain was induced. Collectively, these results illustrate that the functional mouse IL‐2 receptor complex necessarily includes the α chain, and that the regulation of CD8+ T cell growth during immune reaction depends upon α chain expression.
AB - Reconstitution with mouse interleukin‐2 (IL‐2) receptor subunits demonstrated that the mouse IL‐2 receptor complex was different from the human complex in the α chain requirement for the functional mouse receptor complex. The heterotrimeric complex of the mouse exogenous α and β chains and the endogenous γ chain on mouse lymphoid BW5147 cells showed the ability to bind IL‐2 with high affinity, resulting in IL‐2‐induced tyrosine phosphorylation of a cytosolic tyrosine kinase, JAK3, which is involved in IL‐2‐dependent signals. Exogenous introduction of the β chain with the endogenous γ chain, however, could neither confer appreciable IL‐2 binding nor IL‐2‐induced signal transduction on BW5147 cells, unlike the human βγ heterodimer. Mouse spleen CD8+ cells, not having the α chain initially, showed IL‐2‐dependent cell proliferation only when expression of the α chain was induced. Collectively, these results illustrate that the functional mouse IL‐2 receptor complex necessarily includes the α chain, and that the regulation of CD8+ T cell growth during immune reaction depends upon α chain expression.
KW - Interleukin‐2
KW - Interleukin‐2 receptor complex
KW - α chain of interleukin‐2 receptor
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U2 - 10.1002/eji.1830251102
DO - 10.1002/eji.1830251102
M3 - Article
C2 - 7489734
AN - SCOPUS:0028863215
VL - 25
SP - 3001
EP - 3005
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 11
ER -