Difference spectra measurement of squid rhodopsin in the submillimeter wave region

Akio Kishigami; Masatoshi Itoh; Tatsuo Nozokido; Hiroaki Minamide; Yasuo Tsukahara; Koji Mizuno

doi:10.1039/b300197k

Difference spectra measurement of squid rhodopsin in the submillimeter wave region

Akio Kishigami, Masatoshi Itoh, Tatsuo Nozokido, Hiroaki Minamide, Yasuo Tsukahara, Koji Mizuno

Cyclotron and Radioisotope Center (CYRIC)

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Theoretical studies by computer simulation have shown that vibrational modes, which depend on the subdomain motions of proteins, are located in the submillimeter wave region (i.e., 10-100 cm^-1, 0.3-3 THz). We have successfully observed, by measuring the difference spectrum between squid rhodopsin and metarhodopsin to avoid water absorption, that squid rhodopsin shows its absorption features in this region. Our experimental results show that a native protein in solution indicates not only an absorbent property in this region but also an actual change of absorption with changes in protein conformation.

Original language	English
Pages (from-to)	1303-1306
Number of pages	4
Journal	Photochemical and Photobiological Sciences
Volume	2
Issue number	12
DOIs	https://doi.org/10.1039/b300197k
Publication status	Published - 2003 Dec

ASJC Scopus subject areas

Physical and Theoretical Chemistry

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title = "Difference spectra measurement of squid rhodopsin in the submillimeter wave region",

abstract = "Theoretical studies by computer simulation have shown that vibrational modes, which depend on the subdomain motions of proteins, are located in the submillimeter wave region (i.e., 10-100 cm-1, 0.3-3 THz). We have successfully observed, by measuring the difference spectrum between squid rhodopsin and metarhodopsin to avoid water absorption, that squid rhodopsin shows its absorption features in this region. Our experimental results show that a native protein in solution indicates not only an absorbent property in this region but also an actual change of absorption with changes in protein conformation.",

author = "Akio Kishigami and Masatoshi Itoh and Tatsuo Nozokido and Hiroaki Minamide and Yasuo Tsukahara and Koji Mizuno",

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AU - Kishigami, Akio

AU - Itoh, Masatoshi

AU - Nozokido, Tatsuo

AU - Minamide, Hiroaki

AU - Tsukahara, Yasuo

AU - Mizuno, Koji

PY - 2003/12

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