Difference of Epstein-Barr virus isolates from Japanese patients and African Burkitt's lymphoma cell lines based on the sequence of latent membrane protein 1

Kyosuke Kanai, Yukio Satoh, Yuriko Saiki, Haruo Ohtani, Takeshi Sairenji

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Sequence variations in the Epstein-Barr virus (EBV) latent membrane protein 1 (LMP1) gene have been described in many EBV-isolates. To characterize the genomic relationship between Japanese EBV and the EBV isolates of other countries, we analyzed the LMP1 nucleotide sequences in EBV positive cell lines and clinical specimens, including five African Burkitt's lymphoma (BL) cell lines, a Japanese BL cell line, a B-lymphoblastoid cell line, a nasopharyngeal carcinoma hybrid cell line, six gastric carcinoma tissues, two peripheral blood mononuclear cells, and a B95-8 cell line, which contained the prototype EBV genome. We determined the C-terminal nucleotide sequences of LMP1 by PCR-direct sequencing analysis and characterized the sequence variation of Japanese isolates, made a phylogenetic tree from the sequence patterns of LMP1 by a neighbor-joining method. The results indicate that the Japanese EBV isolates are greatly different from the African BL isolates but are closely related to the China 1, which is a strain of Chinese EBV isolates.

Original languageEnglish
Pages (from-to)55-61
Number of pages7
JournalVirus Genes
Volume34
Issue number1
DOIs
Publication statusPublished - 2007 Jan
Externally publishedYes

Keywords

  • Epstein-Barr virus
  • Evolution of EBV
  • Geographical location
  • LMP1 gene
  • Neighbor-joining method
  • Phylogenetic tree

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

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