Difference in urinary LTE4 and 11-dehydro-TXB2 excretion in asthmatic patients

Naoto Suzuki, Takanori Hishinuma, Fumiaki Abe, Ken Omata, Sadayoshi Ito, Masaharu Sugiyama, Michinao Mizugaki

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Bronchoconstrictor cysteinyl leukotrienes (LT) and thromboxane (TX) A2 have been implicated in the pathogenesis of asthma. Determination of urinary leukotriene E4 (LTE4) and 11-dehydro-TXB2 levels are often used to assess cysteinyl LT and TXA2 production in humans. To define the potential role in the pathogenesis of asthma, we investigated the urinary LTE4 and 11-dehydro-TXB2 levels. LTE4 and 11-dehydro-TXB2 levels were determined using liquid chromatography/tandem mass spectrometry (LC/MS) and gas chromatography/mass spectrometry (GC/MS), respectively. Urinary LTE4 levels in asthmatic patients (192 ± 122 pg/mg creatinine, n = 14) were significantly higher (P < 0.005) than those in healthy volunteers (55 ± 16 pg/mg creatinine, n = 13), but no significant difference in 11-dehydro-TXB2 levels was observed. A significant inverse correlation (r = -0.821, P < 0.005) was found between urinary LTE4 levels and the forced expiratory volume in 1 s (FEV1) but no significant correlation was observed between urinary 11-dehydro-TXB2 levels and FEV1. The present findings suggest that cysteinyl LTs play a more important role in the pathogenesis of asthma than TXA2. (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)395-403
Number of pages9
JournalProstaglandins and Other Lipid Mediators
Volume62
Issue number4
DOIs
Publication statusPublished - 2000

Keywords

  • Asthma
  • Leukotrienes E4 (or LTE4)
  • Microdetermination
  • Thromboxane

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Pharmacology
  • Cell Biology

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