TY - JOUR
T1 - Difference in urinary LTE4 and 11-dehydro-TXB2 excretion in asthmatic patients
AU - Suzuki, Naoto
AU - Hishinuma, Takanori
AU - Abe, Fumiaki
AU - Omata, Ken
AU - Ito, Sadayoshi
AU - Sugiyama, Masaharu
AU - Mizugaki, Michinao
N1 - Funding Information:
A part of this study was supported by grants from Uehara Memorial Foundation in Japan, the Health Sciences Research Grants for the Research on Pharmaceutical and Medical Safety from the Ministry of Health and Welfare of Japan, and Grants-in-Aid for the Research on Health Sciences Focusing on Drug Innovation from Japan Health Sciences Foundation.
PY - 2000
Y1 - 2000
N2 - Bronchoconstrictor cysteinyl leukotrienes (LT) and thromboxane (TX) A2 have been implicated in the pathogenesis of asthma. Determination of urinary leukotriene E4 (LTE4) and 11-dehydro-TXB2 levels are often used to assess cysteinyl LT and TXA2 production in humans. To define the potential role in the pathogenesis of asthma, we investigated the urinary LTE4 and 11-dehydro-TXB2 levels. LTE4 and 11-dehydro-TXB2 levels were determined using liquid chromatography/tandem mass spectrometry (LC/MS) and gas chromatography/mass spectrometry (GC/MS), respectively. Urinary LTE4 levels in asthmatic patients (192 ± 122 pg/mg creatinine, n = 14) were significantly higher (P < 0.005) than those in healthy volunteers (55 ± 16 pg/mg creatinine, n = 13), but no significant difference in 11-dehydro-TXB2 levels was observed. A significant inverse correlation (r = -0.821, P < 0.005) was found between urinary LTE4 levels and the forced expiratory volume in 1 s (FEV1) but no significant correlation was observed between urinary 11-dehydro-TXB2 levels and FEV1. The present findings suggest that cysteinyl LTs play a more important role in the pathogenesis of asthma than TXA2. (C) 2000 Elsevier Science Inc.
AB - Bronchoconstrictor cysteinyl leukotrienes (LT) and thromboxane (TX) A2 have been implicated in the pathogenesis of asthma. Determination of urinary leukotriene E4 (LTE4) and 11-dehydro-TXB2 levels are often used to assess cysteinyl LT and TXA2 production in humans. To define the potential role in the pathogenesis of asthma, we investigated the urinary LTE4 and 11-dehydro-TXB2 levels. LTE4 and 11-dehydro-TXB2 levels were determined using liquid chromatography/tandem mass spectrometry (LC/MS) and gas chromatography/mass spectrometry (GC/MS), respectively. Urinary LTE4 levels in asthmatic patients (192 ± 122 pg/mg creatinine, n = 14) were significantly higher (P < 0.005) than those in healthy volunteers (55 ± 16 pg/mg creatinine, n = 13), but no significant difference in 11-dehydro-TXB2 levels was observed. A significant inverse correlation (r = -0.821, P < 0.005) was found between urinary LTE4 levels and the forced expiratory volume in 1 s (FEV1) but no significant correlation was observed between urinary 11-dehydro-TXB2 levels and FEV1. The present findings suggest that cysteinyl LTs play a more important role in the pathogenesis of asthma than TXA2. (C) 2000 Elsevier Science Inc.
KW - Asthma
KW - Leukotrienes E4 (or LTE4)
KW - Microdetermination
KW - Thromboxane
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U2 - 10.1016/S0090-6980(00)00091-5
DO - 10.1016/S0090-6980(00)00091-5
M3 - Article
C2 - 11060902
AN - SCOPUS:0033787420
SN - 0090-6980
VL - 62
SP - 395
EP - 403
JO - Journal of Lipid Mediators
JF - Journal of Lipid Mediators
IS - 4
ER -