TY - JOUR
T1 - Difference in urinary 11-dehydro TXB2 and LTE4 excretion in patients with rheumatoid arthritis
AU - Nakamura, H.
AU - Hishinuma, T.
AU - Suzuki, Naoto
AU - Chiba, S.
AU - Tsukamoto, H.
AU - Takabatake, M.
AU - Sawai, T.
AU - Mitomo, T.
AU - Inoue, H.
AU - Matsumoto, F.
AU - Mizugaki, M.
N1 - Funding Information:
A part of this study was supported by Grants-in-Aid for the Health Science Research Grants for the Research of Pharmaceutical and Medical Safety from the Ministry of Health and Welfare of Japan and Research on Health Sciences Focusing on Drug Innovation from Japan Health Sciences Foundation.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2001
Y1 - 2001
N2 - Thromboxane and leukotrienes have been implicated in inflammation. However, the production level of these eicosanoids in patients with rheumatoid arthritis is still unclarified. In the present study, endogenous synthesis of thromboxane and cysteinyl leukotrienes in patients was investigated. The production of eicosanoids in patients is assessed by measuring stable urinary metabolites, 11-dehydro thromboxane B2 and leukotriene E4, using gas chromatography/selected ion monitoring and liquid chromatography/tandem mass spectrometry. The level of urinary thromboxane in patients was significantly higher than that in healthy volunteers (P > 0.05). Furthermore, we investigated the effects of administered drugs on the production of these eicosanoids. The urinary thromboxane level of the untreated group (1630 ± 613 pg/mg creatinine) was much higher than that of healthy volunteers (342 ± 263 pg/mg creatinine). The level in the group receiving NSAID alone was similar to that in healthy volunteers, and the group receiving steroid alone showed slightly lower thromboxane levels than the untreated group. On the other hand, the leukotriene E4 level in patients (280 ± 360 pg/mg creatinine) was also significantly higher than that in healthy volunteers (59 ± 54 pg/mg creatinine, P >0.05). In particular, the group receiving methotrexate (904 ± 685 pg/mg creatinine) had higher leukotriene levels than not only healthy volunteers but also other medicated groups. These findings demonstrated that endogenous thromboxane and leukotriene production in patients with rheumatoid arthritis are enhanced, and the effects of medication on the production of these eicosanoids differed in thromboxane and leukotriene E4.
AB - Thromboxane and leukotrienes have been implicated in inflammation. However, the production level of these eicosanoids in patients with rheumatoid arthritis is still unclarified. In the present study, endogenous synthesis of thromboxane and cysteinyl leukotrienes in patients was investigated. The production of eicosanoids in patients is assessed by measuring stable urinary metabolites, 11-dehydro thromboxane B2 and leukotriene E4, using gas chromatography/selected ion monitoring and liquid chromatography/tandem mass spectrometry. The level of urinary thromboxane in patients was significantly higher than that in healthy volunteers (P > 0.05). Furthermore, we investigated the effects of administered drugs on the production of these eicosanoids. The urinary thromboxane level of the untreated group (1630 ± 613 pg/mg creatinine) was much higher than that of healthy volunteers (342 ± 263 pg/mg creatinine). The level in the group receiving NSAID alone was similar to that in healthy volunteers, and the group receiving steroid alone showed slightly lower thromboxane levels than the untreated group. On the other hand, the leukotriene E4 level in patients (280 ± 360 pg/mg creatinine) was also significantly higher than that in healthy volunteers (59 ± 54 pg/mg creatinine, P >0.05). In particular, the group receiving methotrexate (904 ± 685 pg/mg creatinine) had higher leukotriene levels than not only healthy volunteers but also other medicated groups. These findings demonstrated that endogenous thromboxane and leukotriene production in patients with rheumatoid arthritis are enhanced, and the effects of medication on the production of these eicosanoids differed in thromboxane and leukotriene E4.
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U2 - 10.1054/plef.2001.0329
DO - 10.1054/plef.2001.0329
M3 - Article
C2 - 11993724
AN - SCOPUS:18344386204
VL - 65
SP - 301
EP - 306
JO - Prostaglandins Leukotrienes and Essential Fatty Acids
JF - Prostaglandins Leukotrienes and Essential Fatty Acids
SN - 0952-3278
IS - 5-6
ER -