TY - JOUR
T1 - Difference in the xylitol sensitivity of acid production among Streptococcus mutans strains, and its biochemical mechanism
AU - Miyasawa-Hori, H.
AU - Aizawa, S.
AU - Takahashi, N.
N1 - Funding Information:
This study was supported in part by the research fellowship (no. 16·3025 to HH) from the Japan Society for the Promotion of Science.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005/9
Y1 - 2005/9
N2 - Although xylitol inhibits the glycolysis of Streptococcus mutans, the degree of inhibition is known to vary among strains. Thus, we studied the biochemical mechanism by which the inhibition varies, using three strains of S. mutans characterized as highly sensitive, moderately sensitive, and resistant to xylitol. The inhibitory effect of xylitol was investigated by measurement of the acid production rate, the metabolic intermediates and the activities of phosphoenolpyruvate phosphotransferase system (PEP-PTS). The results indicate that the higher xylitol: PEP-PTS activity the strain had and the more effectively xylitol inhibited its glucose: PEP-PTS activity, the more sensitive to xylitol the strain was. These observations suggest the following inhibitory mechanisms: (1) direct inhibition of glycolytic enzymes by intracellular accumulation of xylitol 5-phosphate derived from xylitol: PEP-PTS and (2) indirect inhibition through competition for a phosphoryl donor between glucose: and xylitol: PEP-PTS.
AB - Although xylitol inhibits the glycolysis of Streptococcus mutans, the degree of inhibition is known to vary among strains. Thus, we studied the biochemical mechanism by which the inhibition varies, using three strains of S. mutans characterized as highly sensitive, moderately sensitive, and resistant to xylitol. The inhibitory effect of xylitol was investigated by measurement of the acid production rate, the metabolic intermediates and the activities of phosphoenolpyruvate phosphotransferase system (PEP-PTS). The results indicate that the higher xylitol: PEP-PTS activity the strain had and the more effectively xylitol inhibited its glucose: PEP-PTS activity, the more sensitive to xylitol the strain was. These observations suggest the following inhibitory mechanisms: (1) direct inhibition of glycolytic enzymes by intracellular accumulation of xylitol 5-phosphate derived from xylitol: PEP-PTS and (2) indirect inhibition through competition for a phosphoryl donor between glucose: and xylitol: PEP-PTS.
KW - Acid production
KW - Phosphoenolpyruvate phosphotransferase system
KW - Streptococcus mutans
KW - Xylitol
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U2 - 10.1016/j.ics.2005.06.049
DO - 10.1016/j.ics.2005.06.049
M3 - Review article
AN - SCOPUS:33646463023
SN - 0531-5131
VL - 1284
SP - 187
EP - 188
JO - International Congress Series
JF - International Congress Series
ER -