TY - JOUR
T1 - Difference in interleukin-8 transcriptional activity induced in THP-G8 cells by particulate matter collected in winter and summer in western Japan
AU - Watanabe, Masanari
AU - Kurai, Jun
AU - Minato, Sayaka
AU - Noma, Hisashi
AU - Sano, Hiroyuki
AU - Saito, Rumiko
AU - Aiba, Setsuya
AU - Oshimura, Mitsuo
AU - Hatakeyama, Keisuke
AU - Yamasaki, Akira
AU - Shimizu, Eiji
N1 - Publisher Copyright:
© 2015, University of Tokushima. All rights reserved.
PY - 2015/9/18
Y1 - 2015/9/18
N2 - Background: Airborne particulate matter (PM) may stimulate production of pro-inflammatory cytokines, and thus exposure to PM affects pulmonary diseases. However, the effects of PM on pulmonary diseases have differed among studies. Methods: PM was collected from February 2 to 28 (winter) and June 1 to 17 (summer) in 2013 in Yurihama, Japan, using filters to separate particles with different aerodynamic diameters (1.1 to <2.2 μm, 2.2 to <3.3 μm, 3.3 to <7.0 μm, and 7.0 to <10 μm). Interleukin (IL)-8 transcriptional activity in THP-G8 cells was examined in response to winter and summer PM with different diameters. Results: IL-8 transcriptional activity induced by winter PM was significantly higher than that with summer PM for each diameter. For winter PM, IL-8 activity increased with an increased diameter, whereas this activity did not differ between a solvent control and each summer PM diameter. This indicates that summer PM does not stimulate production of IL-8 in THP-G8 cells. Conclusion: The effects of PM on production of pro-inflammatory cytokines differ with the season and PM diameter. This suggests that the compositions of PM differ on season, and the evaluation of compositions of PM is important in understanding the association of health with short-term exposure to PM.
AB - Background: Airborne particulate matter (PM) may stimulate production of pro-inflammatory cytokines, and thus exposure to PM affects pulmonary diseases. However, the effects of PM on pulmonary diseases have differed among studies. Methods: PM was collected from February 2 to 28 (winter) and June 1 to 17 (summer) in 2013 in Yurihama, Japan, using filters to separate particles with different aerodynamic diameters (1.1 to <2.2 μm, 2.2 to <3.3 μm, 3.3 to <7.0 μm, and 7.0 to <10 μm). Interleukin (IL)-8 transcriptional activity in THP-G8 cells was examined in response to winter and summer PM with different diameters. Results: IL-8 transcriptional activity induced by winter PM was significantly higher than that with summer PM for each diameter. For winter PM, IL-8 activity increased with an increased diameter, whereas this activity did not differ between a solvent control and each summer PM diameter. This indicates that summer PM does not stimulate production of IL-8 in THP-G8 cells. Conclusion: The effects of PM on production of pro-inflammatory cytokines differ with the season and PM diameter. This suggests that the compositions of PM differ on season, and the evaluation of compositions of PM is important in understanding the association of health with short-term exposure to PM.
KW - Interleukin-8
KW - Particulate matter
KW - Pro-inflammatory cytokine
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U2 - 10.2152/jmi.62.145
DO - 10.2152/jmi.62.145
M3 - Article
C2 - 26399338
AN - SCOPUS:84942044007
SN - 1343-1420
VL - 62
SP - 145
EP - 148
JO - Journal of Medical Investigation
JF - Journal of Medical Investigation
IS - 3
ER -