Dielectrophoretic Measurement of Bacterial Motor Characteristics

Masao Washizu; Yuichi Kurahashi; Hirokazu Iochi; Osamu Kurosawa; Shin Ichi Aizawa; Hirokazu Hotani; Seishi Kudo; Yukio Magariyama

doi:10.1109/28.216534

Dielectrophoretic Measurement of Bacterial Motor Characteristics

Masao Washizu, Yuichi Kurahashi, Hirokazu Iochi, Osamu Kurosawa, Shin Ichi Aizawa, Hirokazu Hotani, Seishi Kudo, Yukio Magariyama

ENG - Applied Physics

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

Two novel methods of measuring bacterial motor characteristics using ac field effects in a microfabricated electrode system are presented in this paper. One is the measurement of the external force-to-velocity (F-v) characteristics of swimming bacteria. Electrostatic orientation of bacteria parallel to the field lines is used to guide the bacterial locomotion along a line. Then dielectrophoresis is used to apply an external force either forward or backward to the swimming bacteria, and by measuring the velocity of locomotion, the F-v curve is obtained. The other is the measurement of torque-to-speed (T-ω) characteristics of the motor. Electrorotation is used to apply external torque to the tethered cells, and by changing the applied torque and measuring the rotation speed, the T-w curves obtained. The results show that the motor generates approximately constant torque in the measured range of ω (0–100 Hz), regardless of the direction of rotation.

Original language	English
Pages (from-to)	286-294
Number of pages	9
Journal	IEEE Transactions on Industry Applications
Volume	29
Issue number	2
DOIs	https://doi.org/10.1109/28.216534
Publication status	Published - 1993

ASJC Scopus subject areas

Control and Systems Engineering
Industrial and Manufacturing Engineering
Electrical and Electronic Engineering

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10.1109/28.216534

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@article{faec1960a8d74f9d994afc90116cadb1,

title = "Dielectrophoretic Measurement of Bacterial Motor Characteristics",

abstract = "Two novel methods of measuring bacterial motor characteristics using ac field effects in a microfabricated electrode system are presented in this paper. One is the measurement of the external force-to-velocity (F-v) characteristics of swimming bacteria. Electrostatic orientation of bacteria parallel to the field lines is used to guide the bacterial locomotion along a line. Then dielectrophoresis is used to apply an external force either forward or backward to the swimming bacteria, and by measuring the velocity of locomotion, the F-v curve is obtained. The other is the measurement of torque-to-speed (T-ω) characteristics of the motor. Electrorotation is used to apply external torque to the tethered cells, and by changing the applied torque and measuring the rotation speed, the T-w curves obtained. The results show that the motor generates approximately constant torque in the measured range of ω (0–100 Hz), regardless of the direction of rotation.",

author = "Masao Washizu and Yuichi Kurahashi and Hirokazu Iochi and Osamu Kurosawa and Aizawa, {Shin Ichi} and Hirokazu Hotani and Seishi Kudo and Yukio Magariyama",

note = "Funding Information: Paper IUSD 91-84, approved by the Electrostatic Processes Committee of the IEEE Industry Applications Society for presentation at the 1991 IAS Annual Meeting, Dearbom, MI, September 28-October 4. This work was supported by the Ministry of Education of Japan under Grant 02855059 and by Advance Company. Manuscript released for publication August 17, 1992. M. Washizu is with the Department of Electrical Engineering and Electronics, Seikei University, Tokyo, Japan. Y. Kurahashi is with the Department of System Development, QUICK Corporation, Tokyo, Japan. H. Iochi is with the Nikko Securities Company Ltd., Chiba, Japan. 0. Kurosawa is with Advance Company, Tokyo, Japan. S. Aizawa and H. Hotani are with the Department of Bioscience, Teikyo University, Toyosatodai, Utsunomiya, Japan. S. Kudo and Y. Magariyama are with Tsukuba Research Laboratory, Yaskawa Electric Corporation, Tsukuba, Japan. IEEE Log Number 9207103.",

year = "1993",

doi = "10.1109/28.216534",

language = "English",

volume = "29",

pages = "286--294",

journal = "IEEE Transactions on Applications and Industry",

issn = "0093-9994",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

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T1 - Dielectrophoretic Measurement of Bacterial Motor Characteristics

AU - Washizu, Masao

AU - Kurahashi, Yuichi

AU - Iochi, Hirokazu

AU - Kurosawa, Osamu

AU - Aizawa, Shin Ichi

AU - Hotani, Hirokazu

AU - Kudo, Seishi

AU - Magariyama, Yukio

N1 - Funding Information: Paper IUSD 91-84, approved by the Electrostatic Processes Committee of the IEEE Industry Applications Society for presentation at the 1991 IAS Annual Meeting, Dearbom, MI, September 28-October 4. This work was supported by the Ministry of Education of Japan under Grant 02855059 and by Advance Company. Manuscript released for publication August 17, 1992. M. Washizu is with the Department of Electrical Engineering and Electronics, Seikei University, Tokyo, Japan. Y. Kurahashi is with the Department of System Development, QUICK Corporation, Tokyo, Japan. H. Iochi is with the Nikko Securities Company Ltd., Chiba, Japan. 0. Kurosawa is with Advance Company, Tokyo, Japan. S. Aizawa and H. Hotani are with the Department of Bioscience, Teikyo University, Toyosatodai, Utsunomiya, Japan. S. Kudo and Y. Magariyama are with Tsukuba Research Laboratory, Yaskawa Electric Corporation, Tsukuba, Japan. IEEE Log Number 9207103.

PY - 1993

Y1 - 1993

N2 - Two novel methods of measuring bacterial motor characteristics using ac field effects in a microfabricated electrode system are presented in this paper. One is the measurement of the external force-to-velocity (F-v) characteristics of swimming bacteria. Electrostatic orientation of bacteria parallel to the field lines is used to guide the bacterial locomotion along a line. Then dielectrophoresis is used to apply an external force either forward or backward to the swimming bacteria, and by measuring the velocity of locomotion, the F-v curve is obtained. The other is the measurement of torque-to-speed (T-ω) characteristics of the motor. Electrorotation is used to apply external torque to the tethered cells, and by changing the applied torque and measuring the rotation speed, the T-w curves obtained. The results show that the motor generates approximately constant torque in the measured range of ω (0–100 Hz), regardless of the direction of rotation.

AB - Two novel methods of measuring bacterial motor characteristics using ac field effects in a microfabricated electrode system are presented in this paper. One is the measurement of the external force-to-velocity (F-v) characteristics of swimming bacteria. Electrostatic orientation of bacteria parallel to the field lines is used to guide the bacterial locomotion along a line. Then dielectrophoresis is used to apply an external force either forward or backward to the swimming bacteria, and by measuring the velocity of locomotion, the F-v curve is obtained. The other is the measurement of torque-to-speed (T-ω) characteristics of the motor. Electrorotation is used to apply external torque to the tethered cells, and by changing the applied torque and measuring the rotation speed, the T-w curves obtained. The results show that the motor generates approximately constant torque in the measured range of ω (0–100 Hz), regardless of the direction of rotation.

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Dielectrophoretic Measurement of Bacterial Motor Characteristics

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