Dielectrophoretic Measurement of Bacterial Motor Characteristics

Masao Washizu; Yuichi Kurahashi; Hirokazu Iochi; Osamu Kurosawa; Shin Ichi Aizawa; Hirokazu Hotani; Seishi Kudo; Yukio Magariyama

doi:10.1109/28.216534

Dielectrophoretic Measurement of Bacterial Motor Characteristics

Masao Washizu, Yuichi Kurahashi, Hirokazu Iochi, Osamu Kurosawa, Shin Ichi Aizawa, Hirokazu Hotani, Seishi Kudo, Yukio Magariyama

ENG - Applied Physics

Research output: Contribution to journal › Article

52 Citations (Scopus)

Abstract

Two novel methods of measuring bacterial motor characteristics using ac field effects in a microfabricated electrode system are presented in this paper. One is the measurement of the external force-to-velocity (F-v) characteristics of swimming bacteria. Electrostatic orientation of bacteria parallel to the field lines is used to guide the bacterial locomotion along a line. Then dielectrophoresis is used to apply an external force either forward or backward to the swimming bacteria, and by measuring the velocity of locomotion, the F-v curve is obtained. The other is the measurement of torque-to-speed (T-ω) characteristics of the motor. Electrorotation is used to apply external torque to the tethered cells, and by changing the applied torque and measuring the rotation speed, the T-w curves obtained. The results show that the motor generates approximately constant torque in the measured range of ω (0–100 Hz), regardless of the direction of rotation.

Original language	English
Pages (from-to)	286-294
Number of pages	9
Journal	IEEE Transactions on Industry Applications
Volume	29
Issue number	2
DOIs	https://doi.org/10.1109/28.216534
Publication status	Published - 1993 Jan 1

ASJC Scopus subject areas

Control and Systems Engineering
Industrial and Manufacturing Engineering
Electrical and Electronic Engineering

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Washizu, M., Kurahashi, Y., Iochi, H., Kurosawa, O., Aizawa, S. I., Hotani, H., Kudo, S., & Magariyama, Y. (1993). Dielectrophoretic Measurement of Bacterial Motor Characteristics. IEEE Transactions on Industry Applications, 29(2), 286-294. https://doi.org/10.1109/28.216534

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abstract = "Two novel methods of measuring bacterial motor characteristics using ac field effects in a microfabricated electrode system are presented in this paper. One is the measurement of the external force-to-velocity (F-v) characteristics of swimming bacteria. Electrostatic orientation of bacteria parallel to the field lines is used to guide the bacterial locomotion along a line. Then dielectrophoresis is used to apply an external force either forward or backward to the swimming bacteria, and by measuring the velocity of locomotion, the F-v curve is obtained. The other is the measurement of torque-to-speed (T-ω) characteristics of the motor. Electrorotation is used to apply external torque to the tethered cells, and by changing the applied torque and measuring the rotation speed, the T-w curves obtained. The results show that the motor generates approximately constant torque in the measured range of ω (0–100 Hz), regardless of the direction of rotation.",

author = "Masao Washizu and Yuichi Kurahashi and Hirokazu Iochi and Osamu Kurosawa and Aizawa, {Shin Ichi} and Hirokazu Hotani and Seishi Kudo and Yukio Magariyama",

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AU - Kudo, Seishi

AU - Magariyama, Yukio

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N2 - Two novel methods of measuring bacterial motor characteristics using ac field effects in a microfabricated electrode system are presented in this paper. One is the measurement of the external force-to-velocity (F-v) characteristics of swimming bacteria. Electrostatic orientation of bacteria parallel to the field lines is used to guide the bacterial locomotion along a line. Then dielectrophoresis is used to apply an external force either forward or backward to the swimming bacteria, and by measuring the velocity of locomotion, the F-v curve is obtained. The other is the measurement of torque-to-speed (T-ω) characteristics of the motor. Electrorotation is used to apply external torque to the tethered cells, and by changing the applied torque and measuring the rotation speed, the T-w curves obtained. The results show that the motor generates approximately constant torque in the measured range of ω (0–100 Hz), regardless of the direction of rotation.

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