Dictyobacter vulcani sp. Nov., belonging to the class ktedonobacteria, isolated from soil of the mt zao volcano

Yu Zheng, Chiung Mei Wang, Yasuteru Sakai, Keietsu Abe, Akira Yokota, Shuhei Yabe

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    3 Citations (Scopus)


    An aerobic, Gram-stain-positive, mesophilic Ktedonobacteria strain, W12T, was isolated from soil of the Mt Zao volcano in Miyagi, Japan. Cells were filamentous, non-motile, and grew at 20–37 °C (optimally at 30 °C), at pH 5.0–7.0 (optimally at pH 6.0) and with <2 % (w/v) NaCl on 10-fold diluted Reasoner’s 2A (R2A) medium. Oval-shaped spores were formed on aerial mycelia. Strain W12T hydrolysed microcrystalline cellulose and xylan very weakly, and used d-glucose as its sole carbon source. The major menaquinone was MK-9, and the major cellular fatty acids were C16: 1 2-OH, iso-C17: 0, summed feature 9 (10-methyl C16: 0 and/ or iso-C17: 1ω9c) and anteiso-C17: 0. Cell-wall sugars were mannose and xylose, and cell-wall amino acids were d-glutamic acid, glycine, l-serine, d-alanine, l-alanine, β-alanine and l-ornithine. Polar lipids were phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, an unidentified glycolipid and an unidentified phospholipid. Strain W12T has a genome of 7.42 Mb with 49.7 mol% G+C content. Nine copies of 16S rRNA genes with a maximum dissimilarity of 1.02 % and 13 biosynthetic gene clus-ters mainly coding for peptide products were predicted in the genome. Phylogenetic analysis based on both 16S rRNA gene and whole genome sequences indicated that strain W12T represents a novel species in the genus Dictyobacter. The most closely related Dictyobacter type strain was Dictyobacter alpinus Uno16T, with 16S rRNA gene sequence similarity and genomic average nucleotide identity of 98.37 % and 80.00 %, respectively. Herein, we propose the name Dictyobacter vulcani sp. nov. for the type strain W12T (=NBRC 113551T=BCRC 81169T) in the bacterial class Ktedonobacteria.

    Original languageEnglish
    Article number003975
    Pages (from-to)1805-1813
    Number of pages9
    JournalInternational journal of systematic and evolutionary microbiology
    Issue number3
    Publication statusPublished - 2020


    • Class Ktedonobacteria
    • Dictyobacter vulcani sp
    • Nov
    • Polyphasic taxonomy
    • Whole genome sequencing

    ASJC Scopus subject areas

    • Microbiology
    • Ecology, Evolution, Behavior and Systematics


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