TY - JOUR
T1 - Diagnostic value of antibody responses to multiple antigens from Mycobacterium tuberculosis in active and latent tuberculosis
AU - Senoputra, Muhammad Andrian
AU - Shiratori, Beata
AU - Hasibuan, Fakhrial Mirwan
AU - Koesoemadinata, Raspati Cundarani
AU - Apriani, Lika
AU - Ashino, Yugo
AU - Ono, Kenji
AU - Oda, Tetsuya
AU - Matsumoto, Makoto
AU - Suzuki, Yasuhiko
AU - Alisjahbana, Bachti
AU - Hattori, Toshio
N1 - Funding Information:
This work was supported by the Scientific Research Expenses for Health and Welfare program from the Ministry of Health, Labour and Welfare, Japan ; the Science and Technology Research Partnership for Sustainable Development from the Japan Science and Technology Agency, Japan ; Strategic Japanese-Thailand Cooperative Program on Biotechnology, JST ; collaborative funding from the Research Centre for Zoonosis Control at Hokkaido University ; and special research grant from the International Research Institute of Disaster Science of Tohoku University . We are grateful to Kyowa Medex, Japan, for providing Determiner TBGL Antibody ELISA Kit.
PY - 2015/11
Y1 - 2015/11
N2 - We investigated the antibody responses to 10 prospective Mycobacterium tuberculosis (MTB) antigens and evaluated their ability to discriminate between latent (LTBI) and active pulmonary tuberculosis (TB). Our results indicate that plasma levels of anti-α-crystallin (ACR), antilipoarabinomannan, anti-trehalose 6,6'-dimycolate, and anti-tubercular-glycolipid antigen antibodies were higher in patients with active TB, compared to those in the LTBI and control subjects. No differences in the antibodies were observed between the control and LTBI subjects. Antibodies against the glycolipid antigens could not distinguish between Mycobacterium avium complex (MAC)-negative TB patients and MAC-infected LTBI individuals. The most useful serological marker was antibodies to ACR, with MAC-negative TB patients having higher titers than those observed in MAC-positive LTBI and control subjects. Our data indicate that antibody to ACR is a promising target for the serological diagnosis of patients with active TB patients. When dealing with antiglycolipid antibodies, MAC coinfection should always be considered in serological studies.
AB - We investigated the antibody responses to 10 prospective Mycobacterium tuberculosis (MTB) antigens and evaluated their ability to discriminate between latent (LTBI) and active pulmonary tuberculosis (TB). Our results indicate that plasma levels of anti-α-crystallin (ACR), antilipoarabinomannan, anti-trehalose 6,6'-dimycolate, and anti-tubercular-glycolipid antigen antibodies were higher in patients with active TB, compared to those in the LTBI and control subjects. No differences in the antibodies were observed between the control and LTBI subjects. Antibodies against the glycolipid antigens could not distinguish between Mycobacterium avium complex (MAC)-negative TB patients and MAC-infected LTBI individuals. The most useful serological marker was antibodies to ACR, with MAC-negative TB patients having higher titers than those observed in MAC-positive LTBI and control subjects. Our data indicate that antibody to ACR is a promising target for the serological diagnosis of patients with active TB patients. When dealing with antiglycolipid antibodies, MAC coinfection should always be considered in serological studies.
KW - Lipoarabinomannan
KW - Mycolic acid
KW - Tubercular-glycolipid
KW - Tuberculosis
KW - α-Crystallin
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U2 - 10.1016/j.diagmicrobio.2015.07.021
DO - 10.1016/j.diagmicrobio.2015.07.021
M3 - Article
C2 - 26307672
AN - SCOPUS:84943583213
VL - 83
SP - 278
EP - 285
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
SN - 0732-8893
IS - 3
ER -