Diagnostic performance evaluation of sulfate-conjugated cholesterol metabolites as urinary biomarkers of Niemann–Pick disease type C

Masamitsu Maekawa, Aya Narita, Isamu Jinnoh, Takashi Iida, Thorsten Marquardt, Eugen Mengel, Yoshikatsu Eto, Peter T. Clayton, Hiroaki Yamaguchi, Nariyasu Mano

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Background: Niemann–Pick disease type C (NPC) is an autosomal recessive inherited disorder with progressive neuronal degeneration. Because conventional diagnostic methods are complicated and invasive, biomarker tests have drawn attention. We aimed to evaluate three urinary conjugated cholesterol metabolites as diagnostic biomarkers for NPC. Methods: Urine samples from 23 patients with NPC, 28 healthy controls, and 7 patients with inherited metabolic disorders were analyzed. 3β-Sulfooxy-7β-N-acetylglucosaminyl-5-cholen-24-oic acid and its glycine and taurine conjugates in urine were quantified by liquid chromatography-tandem mass spectrometry. The diagnostic performance of the three metabolites and their total concentration was evaluated. Result: Creatinine-corrected concentrations of three metabolites and their total concentration were all significantly higher in NPC patients (0.0098 < P <.0448). The area under the receiver operating curve for all metabolites exceeded 0.95, the clinical specificity was 92–100%, and the clinical sensitivity was ~95%. In the urine of patients with other inherited metabolic diseases, the concentrations of the metabolites were lower than those in the urine of patients with NPC. Conclusion: These conjugated cholesterol metabolites in urine can serve as useful diagnostic markers for noninvasive screening of NPC.

Original languageEnglish
Pages (from-to)58-63
Number of pages6
JournalClinica Chimica Acta
Volume494
DOIs
Publication statusPublished - 2019 Jul

Keywords

  • Biomarkers
  • Liquid chromatography
  • Mass spectrometry
  • Niemann–Pick disease type C
  • Sulfate-conjugated cholesterol metabolites
  • Urine

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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