Diagnostic advantage of double immunohistochemistry using two mutation-specific anti-IDH antibodies (HMab-1 and MsMab-1) in gliomas

Shingo Takano, Yukinari Kato, Tetsuya Yamamoto, Xing Liu, Eiichi Ishikawa, Mika K. Kaneko, Satoshi Ogasawara, Masahide Matsuda, Masayuki Noguchi, Akira Matsumura

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Isocitrate dehydrogenase (IDH) mutation is a valuable prognostic marker and a tool for decision-making for glioma treatment. An algorithm for IDH mutation screening was recently proposed—it consists of a two-step process of an initial search for the most common IDH1-R132H mutation using immunohistochemistry (IHC)-based assay, followed by DNA-based analysis of IHC-negative or -equivocal cases. Here, we report that immunohistochemistry using two mutation-specific anti-IDH monoclonal antibodies (mAbs)—an IDH1-R132H-specific mAb (clone HMab-1) and a multi-specific mAb (clone MsMab-1)—is easy and reliable for IDH mutation screening. We investigated the IDH status of 54 grade III gliomas. For the first screening, we used HMab-1 IHC and for the second, (of HMab-1-negative cases) we used MsMab-1 IHC. The double IHC screening results were confirmed using sequence analysis (100 % specificity and 100 % sensitivity). Thirty of 54 cases (55.6 %) had IDH mutations and the remaining 24 were of the IDH wild type; moreover, the screening results predicted grade III glioma prognosis. IDH sequencing procedures are popular but inconsistent across laboratories. By contrast, double IHC screening using HMab-1 and MsMab-1 is very reliable for detecting IDH1/2 mutations and can predict survival in grade III glioma patients.

Original languageEnglish
Pages (from-to)169-175
Number of pages7
JournalBrain Tumor Pathology
Volume32
Issue number3
DOIs
Publication statusPublished - 2015 Jul 25

Keywords

  • Glioma
  • IDH
  • Immunohistochemistry
  • Monoclonal antibody
  • Mutation

ASJC Scopus subject areas

  • Oncology
  • Clinical Neurology
  • Cancer Research

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