TY - JOUR
T1 - Diagnosis of Parkinson's disease and the level of oxidized DJ-1 protein
AU - Yamagishi, Yuko
AU - Saigoh, Kazumasa
AU - Saito, Yoshiro
AU - Ogawa, Ikuko
AU - Mitsui, Yoshiyuki
AU - Hamada, Yukihiro
AU - Samukawa, Makoto
AU - Suzuki, Hidekazu
AU - Kuwahara, Motoi
AU - Hirano, Makito
AU - Noguchi, Noriko
AU - Kusunoki, Susumu
N1 - Funding Information:
This work was supported in part by the Ministry of Education, Culture, Sports, Science and Technology of Japan (JSPS KAKENHI Grant Number, 26110721 , 25670084 , 16K09705 , and 15H04845 ), Kindai University 21st Century Educational Development Research Grant , and the Practical Research Project for Rare/Intractable Diseases from the Japan Agency for Medical Research and Development, AMED . Appendix A
Publisher Copyright:
© 2017 Elsevier Ireland Ltd and Japan Neuroscience Society
PY - 2018/3
Y1 - 2018/3
N2 - Parkinson's disease (PD) is difficult to distinguish from progressive supranuclear palsy (PSP) and multiple system atrophy (MSA); in addition, biomarker studies in PD mostly focused on those found in the cerebrospinal fluid, and there are few reports of simple biomarkers identified by blood analysis. Previously, the DJ-1 gene was identified as a causative gene of familial PD. Oxidized DJ-1 protein (oxDJ-1) levels were reported to increase in the blood of patients with unmedicated PD. Therefore, we determined the levels of oxDJ-1 in the erythrocytes of patients with PD, PSP, and MSA using ELISA. The oxDJ-1 levels were 165 ± 117, 96 ± 78, and 69 ± 40 ng/mg protein in the PD, PSP, and MSA groups, respectively. The mean level in disease control group was 66 ± 31, revealing significant differences between the PD and PSP groups, the PD and MSA groups, and the PD and disease control groups. Our results indicated that oxDJ-1 levels in erythrocytes can be used as a marker for the differential diagnosis of PD.
AB - Parkinson's disease (PD) is difficult to distinguish from progressive supranuclear palsy (PSP) and multiple system atrophy (MSA); in addition, biomarker studies in PD mostly focused on those found in the cerebrospinal fluid, and there are few reports of simple biomarkers identified by blood analysis. Previously, the DJ-1 gene was identified as a causative gene of familial PD. Oxidized DJ-1 protein (oxDJ-1) levels were reported to increase in the blood of patients with unmedicated PD. Therefore, we determined the levels of oxDJ-1 in the erythrocytes of patients with PD, PSP, and MSA using ELISA. The oxDJ-1 levels were 165 ± 117, 96 ± 78, and 69 ± 40 ng/mg protein in the PD, PSP, and MSA groups, respectively. The mean level in disease control group was 66 ± 31, revealing significant differences between the PD and PSP groups, the PD and MSA groups, and the PD and disease control groups. Our results indicated that oxDJ-1 levels in erythrocytes can be used as a marker for the differential diagnosis of PD.
KW - Biomarker
KW - Erythrocytes
KW - MIBG scintigraphy
KW - MSA
KW - Oxidized DJ-1
KW - PARK7
KW - PD
KW - PSP
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U2 - 10.1016/j.neures.2017.06.008
DO - 10.1016/j.neures.2017.06.008
M3 - Article
C2 - 28705587
AN - SCOPUS:85024091063
VL - 128
SP - 58
EP - 62
JO - Neuroscience Research
JF - Neuroscience Research
SN - 0168-0102
ER -