Diagnosing superinfection keratitis with multiplex polymerase chain reaction

Masaaki Yoshida, Takehiro Hariya, Shunji Yokokura, Kazuichi Maruyama, Kota Sato, Sunao Sugita, Yasuhiro Tomaru, Norio Shimizu, Toru Nakazawa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: To report the potential usefulness of multiplex polymerase chain reaction (mPCR) for diagnosing superinfection keratitis caused by herpes simplex virus-1 (HSV-1), bacteria and fungus. Methods: Case series. Corneal scrapings were analyzed with mPCR for human herpes virus 1–8, bacterial 16S ribosomal DNA (rDNA) and fungal 28S rDNA. Results: Case 1 was a 69-year-old man who presented with refractory infectious keratitis. PCR examination was positive for bacterial 16S rDNA and negative for fungal 28S rDNA. HSV-1 was not examined at this time. A geographic ulcer arose after 2 months of intensive antibacterial treatment. Herpes simplex keratitis (HSK) was suspected; PCR analysis was positive for HSV-1. Corneal scrapings obtained at the initial visit were re-analyzed and found to be HSV-1 positive. Thus, it turned out that this was a case of superinfection keratitis caused by bacteria and HSV-1. Case 2 was a 60-year-old man with corneal ulcer who had received unsuccessful treatment with antibiotics. mPCR analysis was positive for HSV-1, bacterial 16S rDNA and fungal 28S rDNA. The patient was diagnosed with superinfection keratitis caused by HSV-1, bacteria and fungus. Case 3 was an 82-year-old woman who had been treated for HSK and then developed bacterial keratitis during treatment. mPCR analysis was positive for HSV-1 and bacterial 16S rDNA. The patient was diagnosed with superinfection keratitis caused by HSV-1 and bacteria. Conclusion: Superinfection keratitis is hard to diagnose because of its atypical manifestation. mPCR has the potential to allow prompt diagnosis and appropriate treatment in these cases.

Original languageEnglish
Pages (from-to)1004-1008
Number of pages5
JournalJournal of Infection and Chemotherapy
Volume24
Issue number12
DOIs
Publication statusPublished - 2018 Dec

Keywords

  • Bacterial 16S rDNA
  • Fungal 28S rDNA
  • Herpes simplex virus
  • Multiplex polymerase chain reaction
  • Superinfection keratitis

ASJC Scopus subject areas

  • Microbiology (medical)
  • Pharmacology (medical)
  • Infectious Diseases

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