DgMab-6: Antihuman DGKγ Monoclonal Antibody for Immunocytochemistry

Tomoyuki Nakano, Satoshi Ogasawara, Toshiaki Tanaka, Yasukazu Hozumi, Atsumi Yamaki, Fumio Sakane, Yasuhito Shirai, Takuro Nakamura, Miyuki Yanaka, Shinji Yamada, Mika K. Kaneko, Yukinari Kato, Kaoru Goto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Diacylglycerol kinase (DGK) phosphorylates diacylglycerol (DG) to produce phosphatidic acid (PA). Since both DG and PA serve as lipidic second messengers, DGK plays a pivotal role in regulating the balance of two signaling pathways mediated by DG and PA in cellular functions. Reportedly, DGKγ, one of the 10 mammalian DGK isozymes, is involved in leukemic cell differentiation, mast cell function, and membrane traffic. Transfection studies using tagged expression vectors and immunohistochemistry on rat tissues revealed that DGKγ localizes to the cytoplasm, plasma membrane, and Golgi apparatus. However, a limited number of studies reported the detailed localization of native protein of DGKγ in human tissues and cells. In this study, we developed a novel anti-DGKγ monoclonal antibody, DgMab-6, which is very useful in immunocytochemistry of human cultured cells.

Original languageEnglish
Pages (from-to)229-232
Number of pages4
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume37
Issue number5
DOIs
Publication statusPublished - 2018 Nov

Keywords

  • DGKγ
  • immunocytochemical analysis
  • monoclonal antibody

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Nakano, T., Ogasawara, S., Tanaka, T., Hozumi, Y., Yamaki, A., Sakane, F., Shirai, Y., Nakamura, T., Yanaka, M., Yamada, S., Kaneko, M. K., Kato, Y., & Goto, K. (2018). DgMab-6: Antihuman DGKγ Monoclonal Antibody for Immunocytochemistry. Monoclonal antibodies in immunodiagnosis and immunotherapy, 37(5), 229-232. https://doi.org/10.1089/mab.2018.0026