Dextran from Leuconostoc mesenteroides augments immunostimulatory effects by the introduction of phosphate groups

Toshihiro Sato, Junko Nishimura-Uemura, Takeshi Shimosato, Yasushi Kawai, Haruki Kitazawa, Tadao Saito

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39 Citations (Scopus)

Abstract

The immunological effects of phosphorylated dextran (in which phosphate groups were chemically introduced) on murine splenocytes were examined. When dextran produced by Leuconostoc mesenteroides was phosphorylated by a reaction with polyphosphoric acid in formamide solution for 48 h, the degree of phosphorylation of dextran was maximal. The highest phosphorus content (1.7%, wt/wt) was observed in 40 kDa of dextran. The mitogenic response of murine splenocytes was enhanced by the phosphorylated dextran, but its activity was not related to its molecular weight. A strong response was detected at a concentration of 10 to 500 μg/ml, and the highest activity was obtained 48 h after stimulation. Phosphorylated dextran was characterized as a B-cell-specific mitogen. The expressions of CD86 on CD8α-CD11c- and CD8α-CD11c+ cells were augmented by phosphorylated dextran. The levels of mRNA expression of gamma interferon and interleukin-10 on murine splenocytes were also increased by the stimulation. These results demonstrate that dextran exerts immunostimulation by the introduction of phosphate groups.

Original languageEnglish
Pages (from-to)1719-1724
Number of pages6
JournalJournal of Food Protection
Volume67
Issue number8
DOIs
Publication statusPublished - 2004 Aug

ASJC Scopus subject areas

  • Food Science
  • Microbiology

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