Developments in diagnosis for prion diseases

J. Tateishi, T. Kitamoto

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

The protease resistant isoform of prion protein (PrP) is a diagnostic marker of spongiform encephalopathies in humans and animals. Immunoblotting is a sensitive method but requires either fresh or frozen, unfixed materials. Immunohistochemistry using formalin-fixed, paraffin-embedded materials is now also considered to be sensitive and comparable to immunoblotting after various treatments, especially using the hydrolytic autoclaving method on tissue sections before staining. The advantage of this method is that it can be applied to routine pathology materials or long preserved materials. The kuru plaque-type deposition of PrP suggests abnormalities of the PrP gene, while synaptic-type deposition suggests either sporadic CJD or particular familial CJD. PrP gene abnormalities are thus related to PrP deposition and modify clinical symptoms and their progression. A PrP gene analysis can be done using either preclinical, clinical or post-mortem materials.

Original languageEnglish
Pages (from-to)971-979
Number of pages9
JournalBritish Medical Bulletin
Volume49
Issue number4
DOIs
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Medicine(all)

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