Developmental impact of a familial GABAA receptor epilepsy mutation

Cindy Chiu, Christopher A. Reid, Heneu O. Tan, Philip J. Davies, Frank N. Single, Irene Koukoulas, Samuel F. Berkovic, Seong Seng Tan, Rolf Sprengel, Mathew V. Jones, Steven Petrou

Research output: Contribution to journalArticlepeer-review

43 Citations (Scopus)

Abstract

Objective: A major goal of epilepsy research is to understand the molecular and functional basis of seizure genesis. A human GABAA γ2 gene mutation (R43Q) is associated with generalized epilepsy. Introduction of this mutation into a mouse by gene targeting recapitulates the human phenotype demonstrating a strong genotype to phenotype link. GABAA receptors play a role in the moment-to-moment control of brain function and also on the long-term wiring of the brain by directing neuronal development. Our objective was to determine whether developmental expression of the mutation alters seizure susceptibility later in life. Methods: A tetracycline-based conditional model for activation of a hypomorphic Q43 disease allele was created and validated. Seizure susceptibility was assessed using the subcutaneous pentylenetetrazole model. Results: Seizure susceptibility was significantly reduced in mice where the Q43 allele was suppressed during development. Interpretation: These results demonstrate that a human epilepsy-causing mutation impacts network stability during a critical developmental period. These data suggest that identification of presymptomatic children may provide a window for therapeutic intervention before overt symptoms are observed, potentially altering the course of epileptogenesis.

Original languageEnglish
Pages (from-to)284-293
Number of pages10
JournalAnnals of Neurology
Volume64
Issue number3
DOIs
Publication statusPublished - 2008 Sep

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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