Developmental genetic basis for the evolution of pelvic fin loss in the pufferfish Takifugu rubripes

Mikiko Tanaka, Laura A. Hale, Angel Amores, Yi Lin Yan, William A. Cresko, Tohru Suzuki, John H. Postlethwait

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Paired appendages were a key developmental innovation among vertebrates and they eventually evolved into limbs. Ancient developmental control systems for paired fins and limbs are broadly conserved among gnathostome vertebrates. Some lineages including whales, some salamanders, snakes, and many ray-fin fish, independently lost the pectoral, pelvic, or both appendages over evolutionary time. When different taxa independently evolve similar developmental morphologies, do they use the same molecular genetic mechanisms? To determine the developmental genetic basis for the evolution of pelvis loss in the pufferfish Takifugu rubripes (fugu), we isolated fugu orthologs of genes thought to be essential for limb development in tetrapods, including limb positioning (Hoxc6, Hoxd9), limb bud initiation (Pitx1, Tbx4, Tbx5), and limb bud outgrowth (Shh, Fgf10), and studied their expression patterns during fugu development. Results showed that bud outgrowth and initiation fail to occur in fugu, and that pelvis loss is associated with altered expression of Hoxd9a, which we show to be a marker for pelvic fin position in three-spine stickleback Gasterosteus aculeatus. These results rule out changes in appendage outgrowth and initiation genes as the earliest developmental defect in pufferfish pelvic fin loss and suggest that altered Hoxd9a expression in the lateral mesoderm may account for pelvis loss in fugu. This mechanism appears to be different from the mechanism for pelvic loss in stickleback, showing that different taxa can evolve similar phenotypes by different mechanisms.

Original languageEnglish
Pages (from-to)227-239
Number of pages13
JournalDevelopmental Biology
Volume281
Issue number2
DOIs
Publication statusPublished - 2005 May 15

Keywords

  • Evolution of development
  • Fin development
  • Fugu
  • Gasterosteus aculeatus
  • Hox genes
  • Limb initiation
  • Limb positioning
  • Stickleback
  • Takifugu rubripes

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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    Tanaka, M., Hale, L. A., Amores, A., Yan, Y. L., Cresko, W. A., Suzuki, T., & Postlethwait, J. H. (2005). Developmental genetic basis for the evolution of pelvic fin loss in the pufferfish Takifugu rubripes. Developmental Biology, 281(2), 227-239. https://doi.org/10.1016/j.ydbio.2005.02.016