Developmental changes of poly(ADP-ribose) polymerase expression in Sarcophaga peregrina

Mitsuko Masutani, Miyoko Ikejima, Sofia Mariotto, Tadashige Nozaki, Shoichiro Kurata, Shunji Natori, Hiroyasu Esumi, Takashi Sugimura

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

To elucidate the physiological role of poly(ADP-ribose) polymerase (PARP), we studied the levels of PARP mRNA and protein during the developmental stages of Sarcophaga peregrina. PARP mRNA expression changed remarkably throughout the developmental stages. The level of PARP mRNA (the molecular ratio of PARP mRNA to the total RNA) was highest in unfertilized eggs and that of PARP protein (the molecular ratio of PARP protein to the total protein of the crude extract) was high in unfertilized and fertilized eggs and in 1st instar larvae. During the embryogenesis period, the levels of PARP mRNA and protein gradually decreased. The levels of PARP mRNA during larval and pupal periods became less than about 5% of that in unfertilized eggs. After the emergence of adult flies, the levels of PARP mRNA and protein increased both in female and male flies. PARP activity normalized with the total amount of protein in the crude extract changed in parallel to the level of PARP protein throughout the developmental stages. The biological significance of the drastic change of mRNA and protein levels of PARP still remains to be clarified.

Original languageEnglish
Pages (from-to)336-341
Number of pages6
JournalProceedings of the Japan Academy Series B: Physical and Biological Sciences
Volume80
Issue number7
DOIs
Publication statusPublished - 2004 Sep
Externally publishedYes

Keywords

  • Development
  • Expression
  • PARP activity
  • Poly(ADP-ribose) polymerase (PARP)
  • Sarcophaga

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Physics and Astronomy(all)

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