Developmental and nutritional regulation of isoflavone secretion from soybean roots

Akifumi Sugiyama, Yumi Yamazaki, Kazuaki Yamashita, Seiji Takahashi, Toru Nakayama, Kazufumi Yazaki

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Isoflavones play important roles in plant-microbe interactions in rhizospheres. Soybean roots secrete daidzein and genistein to attract rhizobia. Despite the importance of isoflavones in plant-microbe interactions, little is known about the developmental and nutritional regulation of isoflavone secretion from soybean roots. In this study, soybeans were grown in hydroponic culture, and isoflavone contents in tissues, isoflavone secretion from the roots, and the expression of isoflavone conjugates hydrolyzing beta-glucosidase (ICHG) were investigated. Isoflavone contents did not show strong growth-dependent changes, while secretion of daidzein from the roots dramatically changed, with higher secretion during vegetative stages. Coordinately, the expression of ICHG also peaked at vegetative stages. Nitrogen deficiency resulted in 8- and 15-fold increases in secretion of daidzein and genistein, respectively, with no induction of ICHG. Taken together, these results suggest that large amounts of isoflavones were secreted during vegetative stages via the hydrolysis of (malonyl)glucosides with ICHG.

Original languageEnglish
Pages (from-to)89-94
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume80
Issue number1
DOIs
Publication statusPublished - 2016 Jan 1

Keywords

  • Beta-glucosidase
  • Isoflavone secretion
  • Root exudates
  • Soybean

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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