Developmental analysis of the early steps in strigolactone-mediated axillary bud dormancy in rice

Le Luo, Megumu Takahashi, Hiromu Kameoka, Ruyi Qin, Toshihide Shiga, Yuri Kanno, Mitsunori Seo, Masaki Ito, Guohua Xu, Junko Kyozuka

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

By contrast with rapid progress in understanding the mechanisms of biosynthesis and signaling of strigolactone (SL), mechanisms by which SL inhibits axillary bud outgrowth are less well understood. We established a rice (Oryza sativa L.) hydroponic culture system to observe axillary buds at the critical point when the buds enter the dormant state. In situ hybridization analysis indicated that cell division stops in the leaf primordia of the buds entering dormancy. We compared transcriptomes in the axillary buds isolated by laser capture microdissection before and after entering the dormant state and identified genes that are specifically upregulated or downregulated in dormant buds respectively, in SL-mediated axillary bud dormancy. Typically, cell cycle genes and ribosomal genes are included among the active genes while abscisic acid (ABA)-inducible genes are among the dormant genes. Application of ABA to the hydroponic culture suppressed the growth of axillary buds of SL mutants to the same level as wild-type (WT) buds. Tiller number was decreased in the transgenic lines overexpressing OsNCED1, the gene that encodes ABA biosynthesis enzyme. These results indicated that the main site of SL function is the leaf primordia in the axillary bud and that ABA is involved in SL-mediated axillary bud dormancy.

Original languageEnglish
Pages (from-to)1006-1021
Number of pages16
JournalPlant Journal
Volume97
Issue number6
DOIs
Publication statusPublished - 2019 Mar

Keywords

  • Oryza sativa
  • axillary bud
  • development
  • dormancy
  • strigolactone
  • transcriptome analysis

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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