TY - JOUR
T1 - Developmental Ability of Trophoblast Stem Cells in Uniparental Mouse Embryos
AU - Ogawa, H.
AU - Shindo, N.
AU - Kumagai, T.
AU - Usami, Y.
AU - Shikanai, M.
AU - Jonwn, K.
AU - Fukuda, A.
AU - Kawahara, M.
AU - Sotomaru, Y.
AU - Tanaka, S.
AU - Arima, T.
AU - Kono, T.
N1 - Funding Information:
The authors would like to thank Izumi Takahashi and Shoji Tane for their helpful technical assistance and David G. Simmons and Yayoi Obata for their valuable comments on the manuscript. This work was supported by grants from the Bio-oriented Technology Research Advancement Institution (BRAIN), Japan, and the Ministry of Education, Culture, Sports, Science, and Technology, Japan (No. 17780211).
PY - 2009/5
Y1 - 2009/5
N2 - Neither parthenogenetic (PG) nor androgenetic (AG) mouse embryos survive after day 9.5 of pregnancy, owing to the inadequate growth of extraembryonic tissues, including the placenta. At day 9.5 of pregnancy, the placental structures are poorly developed in PG embryos, while trophoblast giant cells are abundant at the implantation site in AG embryos. These findings suggest that both parental genomes are required for placental development. To gain further insight into the trophoblast lineage in PG and AG embryos, we attempted to derive trophoblast stem (TS)-like cell lines from uniparental embryos. Furthermore, we sought to assess their ability to differentiate into cells of the trophoblast lineage by using gene expression analysis. Three cell lines that expressed marker genes for undifferentiated TS cells (Cdx2 and Errβ) were derived from AG embryos. Under differentiation conditions, these cells expressed the trophoblast giant cell-specific genes, but did not express the spongiotrophoblast-specific genes. In contrast, none of the four cell lines from PG embryos expressed marker genes for undifferentiated TS cells, but they expressed Oct3/4, a marker gene for embryonic stem cells. Immunohistochemical analysis indicated that PG blastocysts expressed Oct3/4 and Cdx2 specifically in inner cell mass and the trophectoderm respectively. These results suggest that PG embryos do not possess TS cells, because of the lack of the developmental ability of trophoblast cells.
AB - Neither parthenogenetic (PG) nor androgenetic (AG) mouse embryos survive after day 9.5 of pregnancy, owing to the inadequate growth of extraembryonic tissues, including the placenta. At day 9.5 of pregnancy, the placental structures are poorly developed in PG embryos, while trophoblast giant cells are abundant at the implantation site in AG embryos. These findings suggest that both parental genomes are required for placental development. To gain further insight into the trophoblast lineage in PG and AG embryos, we attempted to derive trophoblast stem (TS)-like cell lines from uniparental embryos. Furthermore, we sought to assess their ability to differentiate into cells of the trophoblast lineage by using gene expression analysis. Three cell lines that expressed marker genes for undifferentiated TS cells (Cdx2 and Errβ) were derived from AG embryos. Under differentiation conditions, these cells expressed the trophoblast giant cell-specific genes, but did not express the spongiotrophoblast-specific genes. In contrast, none of the four cell lines from PG embryos expressed marker genes for undifferentiated TS cells, but they expressed Oct3/4, a marker gene for embryonic stem cells. Immunohistochemical analysis indicated that PG blastocysts expressed Oct3/4 and Cdx2 specifically in inner cell mass and the trophectoderm respectively. These results suggest that PG embryos do not possess TS cells, because of the lack of the developmental ability of trophoblast cells.
KW - Androgenetic
KW - Mouse placenta
KW - Parthenogenetic
KW - Trophoblast stem (TS) cells
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U2 - 10.1016/j.placenta.2009.02.006
DO - 10.1016/j.placenta.2009.02.006
M3 - Article
C2 - 19345411
AN - SCOPUS:65049088616
VL - 30
SP - 448
EP - 456
JO - Placenta
JF - Placenta
SN - 0143-4004
IS - 5
ER -