Development of UV thermal lens microscope (UV-TLM) for ultrasensitive and direct detection of non-labeled biomolecules on a microchip

Manabu Tokeshi; Shinichiro Hiki; Kazuma Mawatari; Akihide Hibara; Takehiko Kitamori

Development of UV thermal lens microscope (UV-TLM) for ultrasensitive and direct detection of non-labeled biomolecules on a microchip

Manabu Tokeshi, Shinichiro Hiki, Kazuma Mawatari, Akihide Hibara, Takehiko Kitamori

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This paper describes the development of an ultraviolet thermal lens microscope (UVTLM) that can detect directly non-labeled biomolecules in microchips. The performance of the UV-TLM was evaluated by the limit of detection (LOD) using standard solution of adenine. The LOD for adenine was calculated to be 1.4×10^-8 M. This sensitivity was 100 times higher than that obtained by conventional spectrophotometry.

Original language	English
Title of host publication	Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Transducer Research Foundation
Pages	452-454
Number of pages	3
ISBN (Print)	0974361119, 9780974361116
Publication status	Published - 2005
Externally published	Yes
Event	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States Duration: 2005 Oct 9 → 2005 Oct 13

Publication series

Name	Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume	1

Other

Other	9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
Country/Territory	United States
City	Boston, MA
Period	05/10/9 → 05/10/13

Keywords

Non-labeled biomolecules
Quasi-continuous wave
Thermal lens microscope
Ultraviolet pulse laser

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Tokeshi, M., Hiki, S., Mawatari, K., Hibara, A., & Kitamori, T. (2005). Development of UV thermal lens microscope (UV-TLM) for ultrasensitive and direct detection of non-labeled biomolecules on a microchip. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 452-454). (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences; Vol. 1). Transducer Research Foundation.

Development of UV thermal lens microscope (UV-TLM) for ultrasensitive and direct detection of non-labeled biomolecules on a microchip. / Tokeshi, Manabu; Hiki, Shinichiro; Mawatari, Kazuma; Hibara, Akihide; Kitamori, Takehiko.

Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Transducer Research Foundation, 2005. p. 452-454 (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences; Vol. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Tokeshi, M, Hiki, S, Mawatari, K, Hibara, A & Kitamori, T 2005, Development of UV thermal lens microscope (UV-TLM) for ultrasensitive and direct detection of non-labeled biomolecules on a microchip. in Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, vol. 1, Transducer Research Foundation, pp. 452-454, 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005, Boston, MA, United States, 05/10/9.

Tokeshi M, Hiki S, Mawatari K, Hibara A, Kitamori T. Development of UV thermal lens microscope (UV-TLM) for ultrasensitive and direct detection of non-labeled biomolecules on a microchip. In Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Transducer Research Foundation. 2005. p. 452-454. (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Tokeshi, Manabu ; Hiki, Shinichiro ; Mawatari, Kazuma ; Hibara, Akihide ; Kitamori, Takehiko. / Development of UV thermal lens microscope (UV-TLM) for ultrasensitive and direct detection of non-labeled biomolecules on a microchip. Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Transducer Research Foundation, 2005. pp. 452-454 (Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

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abstract = "This paper describes the development of an ultraviolet thermal lens microscope (UVTLM) that can detect directly non-labeled biomolecules in microchips. The performance of the UV-TLM was evaluated by the limit of detection (LOD) using standard solution of adenine. The LOD for adenine was calculated to be 1.4×10-8 M. This sensitivity was 100 times higher than that obtained by conventional spectrophotometry.",

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Development of UV thermal lens microscope (UV-TLM) for ultrasensitive and direct detection of non-labeled biomolecules on a microchip

Abstract

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Keywords

ASJC Scopus subject areas

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