Development of the binding molecules for the RNA higher-order structures based on the guanine-recognition by the G-clamp

Hirotaka Murase, Fumi Nagatsugi

Research output: Contribution to journalArticlepeer-review

Abstract

RNA higher-order structures play an important role for control of the gene expression, and the small molecules binding to these structures have potential to act as interfering agents in the RNA-mediated-pathway. In this study, we synthesized new RNA binding molecules based on the G-clamp structure and evaluated their binding properties using the model RNA. The monomeric G-clamp ligand exhibited a fluorescence quenching with RNA-binding. The dimeric G-clamp ligand showed a significant fluorescence OFF/ON response to the RNA hairpin structure containing the guanines, indicating a high affinity of the G-clamp dimer to two neighboring guanines located in the RNA hairpin loop.

Original languageEnglish
Pages (from-to)1320-1324
Number of pages5
JournalBioorganic and Medicinal Chemistry Letters
Volume29
Issue number11
DOIs
Publication statusPublished - 2019 Jun 1

Keywords

  • Fluorescence
  • G-clamp
  • RNA higher-order structures
  • RNA-binding small molecules
  • miRNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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