A Si opto-neural probe having multiple waveguides and metal cover have been developed for the first time. This neural probe consists of 16 recording sites, 3 optical waveguides, and metal waveguide cover for light leakage suppression. Electrochemical properties of the recording sites were carefully evaluated, and it was indicated that the Si opto-neural probe had appropriate characteristics for neuronal recording. The optical stimulation to the neurons expressing ChR2 were also demonstrated using the Si opto-neural probe. As a result, multisite optical stimulations were successfully achieved without light leakage from the optical waveguide because of the metal cover. From in vivo experiments, optically modulated local field potentials (LFPs) were also perfectly recorded using the Si opto-neural probe with three optical waveguides. In addition, current source density (CSD) analysis was performed to the recorded LFPs, leading to that light induced membrane current sank in locally stimulated area. The Si opto-neural probe with multiple optical waveguides and metal-cover is one of the most versatile tools for optogenetics.
|Journal||Transactions of Japanese Society for Medical and Biological Engineering|
|Publication status||Published - 2014 Aug 17|
ASJC Scopus subject areas
- Biomedical Engineering