Development of Si neural probe with optical waveguide for highly accurate optical stimulation of neuron

R. Kobayashi, S. Kanno, S. Sakai, S. Lee, M. Koyanagi, H. Yao, T. Tanaka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

10 Citations (Scopus)

Abstract

Lots of researchers take great interests in brain science. In this area, measurement devices of the brain have been developed by number of groups, and played an important role. Recently, for neuroengineering applications such as optogenetics, optical stimulation devices which consisted of optical fibers have been reported, and used to deliver light to neurons expressing light sensitive channel proteins. However, accurate stimulation of neurons could not be achieved by using these devices because of optical fibers with a diameter of over 100 m. Here, we have proposed a novel Si neural probe with micromachined optical waveguide for multiple and precise optical stimulations of neurons. SiN film was employed as the optical waveguide core due to its optical transmission characteristics. Both the light propagation in the optical waveguide and controllability of output patterns of the light were clearly confirmed by optical experiments using a blue laser. In vitro experiments of optical stimulation of neurons using the fabricated Si neural probe were performed. A CA1 area of a thin hippocampal slice obtained from the brain of a transgenic rat expressing Channelrhodopsin-2 (ChR2) was employed. We stimulated neurons optically using the Si neural probe and successfully observed increases of firing rates in neurons accordingly with the light exposure.

Original languageEnglish
Title of host publication2011 5th International IEEE/EMBS Conference on Neural Engineering, NER 2011
Pages294-297
Number of pages4
DOIs
Publication statusPublished - 2011 Jul 20
Event2011 5th International IEEE/EMBS Conference on Neural Engineering, NER 2011 - Cancun, Mexico
Duration: 2011 Apr 272011 May 1

Publication series

Name2011 5th International IEEE/EMBS Conference on Neural Engineering, NER 2011

Other

Other2011 5th International IEEE/EMBS Conference on Neural Engineering, NER 2011
CountryMexico
CityCancun
Period11/4/2711/5/1

ASJC Scopus subject areas

  • Neuroscience(all)

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