TY - GEN
T1 - Development of S tester lines of Brassica oleracea, Brassica rapa and Raphanus sativus as genetic resources
AU - Nishio, Takeshi
AU - Izumida, Atsushi
AU - Hanzawa, Hideaki
AU - Sakamoto, Koji
PY - 2006/4
Y1 - 2006/4
N2 - The number of S haplotypes, which are estimated to be 50 in Brassica oleracea and more than 100 in Brassica rapa, can be utilized as a criterion to assess the genetic diversity of each accession within genetic resource collections, and can be lost most readily from genetic resource accessions. The finding that the recognition specificities of pollen and stigma are determined by SP11/SCR and SRK, respectively, suggests the difficulties for generation of a new S haplotype. The self-incompatibility system is utilized for the breeding of F1 hybrid cultivars of the vegetables in B. oleracea, B. rapa, and R. sativus. In F1 hybrid breeding using the self-incompatibility system, stability or strength of self-incompatibility, which sometimes depends on the S haplotypes, is required. Therefore, it is important to maintain a large number of S haplotypes as genetic resources. We are now developing a new series of S tester lines of B. oleracea, B. rapa, and R. sativus using molecular techniques for S-haplotype identification. These methods include PCR-RFLP of SLG and SRK and dot blot analysis of SP11/SCR.
AB - The number of S haplotypes, which are estimated to be 50 in Brassica oleracea and more than 100 in Brassica rapa, can be utilized as a criterion to assess the genetic diversity of each accession within genetic resource collections, and can be lost most readily from genetic resource accessions. The finding that the recognition specificities of pollen and stigma are determined by SP11/SCR and SRK, respectively, suggests the difficulties for generation of a new S haplotype. The self-incompatibility system is utilized for the breeding of F1 hybrid cultivars of the vegetables in B. oleracea, B. rapa, and R. sativus. In F1 hybrid breeding using the self-incompatibility system, stability or strength of self-incompatibility, which sometimes depends on the S haplotypes, is required. Therefore, it is important to maintain a large number of S haplotypes as genetic resources. We are now developing a new series of S tester lines of B. oleracea, B. rapa, and R. sativus using molecular techniques for S-haplotype identification. These methods include PCR-RFLP of SLG and SRK and dot blot analysis of SP11/SCR.
KW - F hybrid breeding
KW - Genetic diversity
KW - Identification techniques
KW - S haplotypes
KW - SP11/SCR
KW - SRK
KW - Self-incompatibility
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U2 - 10.17660/ActaHortic.2006.706.15
DO - 10.17660/ActaHortic.2006.706.15
M3 - Conference contribution
AN - SCOPUS:33745103099
SN - 906605445X
SN - 9789066054455
T3 - Acta Horticulturae
SP - 141
EP - 144
BT - Proceedings of the Joint Meeting of the XIVth Crucifer Genetics Workshop and IVth ISHS Symposium on Brassicas
PB - International Society for Horticultural Science
ER -