Development of RAP Tag, a Novel Tagging System for Protein Detection and Purification

Yuki Fujii, Mika K. Kaneko, Satoshi Ogasawara, Shinji Yamada, Miyuki Yanaka, Takuro Nakamura, Noriko Saidoh, Kanae Yoshida, Ryusuke Honma, Yukinari Kato

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Affinity tag systems, possessing high affinity and specificity, are useful for protein detection and purification. The most suitable tag for a particular purpose should be selected from many available affinity tag systems. In this study, we developed a novel affinity tag called the "RAP tag" system, which comprises a mouse antirat podoplanin monoclonal antibody (clone PMab-2) and the RAP tag (DMVNPGLEDRIE). This system is useful not only for protein detection in Western blotting, flow cytometry, and sandwich enzyme-linked immunosorbent assay, but also for protein purification.

Original languageEnglish
Pages (from-to)68-71
Number of pages4
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume36
Issue number2
DOIs
Publication statusPublished - 2017 Apr

Keywords

  • RAP tag
  • affinity tag
  • monoclonal antibody
  • podoplanin
  • protein purification

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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