Development of protein-labeling probes with a redesigned fluorogenic switch based on intramolecular association for no-wash live-cell imaging

Yuichiro Hori, Kyohei Nakaki, Motoki Sato, Shin Mizukami, Kazuya Kikuchi

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

Turn on the switch: Fluorogenic probes for protein labeling based on the photoactive yellow protein (PYP) tag were developed. The fluorescence of the probes is turned off by intramolecular association and switched on by the reversal of this interaction upon reaction with the PYP tag that is fused to the protein of interest (POI, see scheme). The rapid and specific labeling reaction enabled the imaging of cell-surface proteins without washing.

Original languageEnglish
Pages (from-to)5611-5614
Number of pages4
JournalAngewandte Chemie - International Edition
Volume51
Issue number23
DOIs
Publication statusPublished - 2012 Jun 4
Externally publishedYes

Keywords

  • fluorescence imaging
  • fluorescent probes
  • intramolecular association
  • protein modifications
  • protein tags

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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