Development of novel C-nucleoside analogues for the formation of antiparallel-type triplex DNA with duplex DNA that includes TA and dUA base pairs

Yosuke Taniguchi, Yuya Magata, Takayuki Osuki, Ryotaro Notomi, Lei Wang, Hidenori Okamura, Shigeki Sasaki

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Expansion of the triplex DNA forming sequence is required in the genomic targeting fields. Basically, triplex DNA is formed by the interaction between the triplex-forming oligonucleotides and homo-purine region with the target duplex DNA. The presence of the base pair conversion sites hampers stable triplex formation. To overcome this limitation, it is necessary to develop an artificial nucleic acid to recognize the base conversion sites, and the CG and TA base pairs. We describe the synthesis of C-nucleoside analogues and an evaluation of the ability of triplex formation. Consequently, the combined use of the novel C-nucleoside analogues, AY-AY-d(Y-NH2), AY-d(Y-Cl) and IAP-d(Y-Cl), is capable of recognizing duplex DNA including the TA or dUA base pair.

Original languageEnglish
Pages (from-to)2845-2851
Number of pages7
JournalOrganic and Biomolecular Chemistry
Volume18
Issue number15
DOIs
Publication statusPublished - 2020 Apr 21
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

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