Development of normal mice from metaphase I oocytes fertilized with primary spermatocytes

Atsuo Ogura, Osamu Suzuki, Kentaro Tanemura, Keiji Mochida, Yoshiro Kobayashi, Junichiro Matsuda

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72 Citations (Scopus)

Abstract

Primary spermatocytes are the male germ cells before meiosis I. To examine whether these 4n diploid cells are genetically competent to fertilize oocytes and support full embryo development, we introduced the nuclei of pachytene/diplotene spermatocytes into oocytes that were arrested in prophase I (germinal vesicle stage), metaphase I, or metaphase II (Met II). Both the paternal and maternal chromosomes then were allowed to undergo meiosis synchronously until Met II. In the first and second groups, the paternal and maternal chromosomes had intermingled to form a large Met II plate, which was then transferred into a fresh enucleated Met II oocyte. In the third group, the paternal Met II chromosomes were obtained by transferring spermatocyte nuclei into Met II oocytes twice. After activation of the Met II oocytes that were produced, those microfertilized at metaphase I showed the best developmental ability in vitro, and three of these embryos developed into full-term offspring after embryo transfer. Two pups (one male and one female) were proven to be fertile. This finding provides direct evidence that the nuclei of male germ cells acquire the ability to fertilize oocytes before the first meiotic division.

Original languageEnglish
Pages (from-to)5611-5615
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number10
DOIs
Publication statusPublished - 1998 May 12
Externally publishedYes

ASJC Scopus subject areas

  • General

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