Development of multipurpose recombinant reporter bovine leukemia virus

Hironobu Murakami, Yusuke Yajima, Fumiaki Sato, Shinji Kamisuki, Satoshi Taharaguchi, Ken Onda, Sanggun Roh, Jumpei Uchiyama, Masahiro Sakaguchi, Kenji Tsukamoto

Research output: Contribution to journalArticlepeer-review

Abstract

Bovine leukemia virus (BLV) is a global problem that results in significant economic losses to the livestock industry. We developed three virus strains by inserting the HiBiT reporter tag from NanoLuc luciferase (NLuc) into limited sites within BLV molecular clones. Initial analysis for site selection of the tag insertion revealed a permissible site immediately downstream of the viral envelope gene. Therefore, NLuc activity could be used to measure virus copy numbers in the supernatant and the levels of cell infection. Productivity and growth kinetics of the reporter virus were similar to those of the wild-type strain; therefore, the reporter virus can be used to characterize the replication of chimeric viruses as well as responses to the antiviral drug, amprenavir. Collectively, our results suggest that the BLV reporter virus with a HiBiT tag insertion is a highly versatile system for various purposes such as evaluating virus replication and antiviral drugs.

Original languageEnglish
Pages (from-to)226-235
Number of pages10
JournalVirology
Volume548
DOIs
Publication statusPublished - 2020 Sep

Keywords

  • Antiviral drug
  • Bovine leukemia virus
  • Molecular clone
  • Reporter virus
  • Viral replication

ASJC Scopus subject areas

  • Virology

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