Development of mPMab-1, a Mouse-Rat Chimeric Antibody Against Mouse Podoplanin

Shinji Yamada, Mika K. Kaneko, Takuro Nakamura, Osamu Ichii, Satoru Konnai, Yukinari Kato

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Podoplanin (PDPN), the ligand of C-type lectin-like receptor-2, is used as a lymphatic endothelial marker. We previously established clone PMab-1 of rat IgG2a as a specific monoclonal antibody (mAb) against mouse PDPN. PMab-1 is also very sensitive in immunohistochemical analysis; however, rat mAbs seem to be unfavorable for pathologists because anti-mouse IgG and anti-rabbit IgG are usually used as secondary antibodies in commercially available kits for immunohistochemical analysis. In this study, we develop a mouse-rat chimeric antibody, mPMab-1 of mouse IgG2a, which was derived from rat PMab-1 mAb. Immunohistochemical analysis shows that mPMab-1 detects podocytes of the kidney, lymphatic endothelial cells of the colon, and type I alveolar cells of the lung. Importantly, mPMab-1 is more sensitive than PMab-1. This conversion strategy from rat mAb to mouse mAb could be applicable to other mAbs.

Original languageEnglish
Pages (from-to)77-79
Number of pages3
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume36
Issue number2
DOIs
Publication statusPublished - 2017 Apr

Keywords

  • PMab-1
  • chimeric antibody
  • monoclonal antibody
  • podoplanin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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