Development of microsatellite markers for the endangered semi-shrub Chimaphila umbellata (Ericaceae)

Ai Kikuchi, Taisuke Miyazaki, Masayuki Maki

Research output: Contribution to journalComment/debatepeer-review

Abstract

Based on the DNA sequencing reads obtained using 454 pyrosequencing, primers amplifying 16 microsatellite loci were developed for the endangered semi-shrub Chimaphila umbellata, which occurs sporadically in the Japanese Archipelago. These 16 loci were polymorphic in the populations sampled from the Hokkaido and Tohoku Districts; the mean number of alleles was 3.31 and 3.44, and the mean expected heterozygosity was 0.42 and 0.44, respectively. These loci were not linked to each other and contained no null alleles. Amplification using these primers was also tested in the congeneric species C. japonica, but only three of them successfully amplified DNA of the species. These markers will be used to examine genetic diversity and genetic differentiation in populations of C. umbellata.

Original languageEnglish
Pages (from-to)140-143
Number of pages4
JournalPlant Species Biology
Volume33
Issue number2
DOIs
Publication statusPublished - 2018 Apr

Keywords

  • Chimaphila japonica
  • Chimaphila umbellate
  • clonal propagation
  • conservation genetics
  • endangered species
  • genetic diversity

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Plant Science

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