Development of microsatellite markers for the endangered orchid calanthe izu-insularis (Orchidaceae)

Naoyuki Nakahama, Azusa Ito, Shingo Kaneko, Yu Matsuki, Yoshihisa Suyama, Azusa Hayano, Miho Murayama, Yuji Isagi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Microsatellite markers were developed for the endangered orchid Calanthe izu-insularis (Orchidaceae). This species is unique to the Izu Islands in Japan. Unfortunately, its population size has decreased because of excessive collection for horticultural purposes. In addition, although natural hybridization between C. izu-insularis and C. discolor var. discolor has been reported, morphological differences between C. izu-insularis and the hybridized individuals remain unclear. Using next-generation sequencing, 11 polymorphic microsatellite markers were developed. All developed markers could amplify C. aristulifera and nine markers could amplify C. d. var. discolor, two other orchid species that are also endangered in Japan. The number of alleles and expected heterozygosity at each locus were 1–6 (mean, 2.35) and 0.00–0.79 (mean, 0.30), respectively. These microsatellite markers will help conservation geneticists in their investigation of the proportion of pure C. izu-insularis individuals in the Izu Islands.

Original languageEnglish
Pages (from-to)31-35
Number of pages5
JournalGenes and Genetic Systems
Issue number1
Publication statusPublished - 2018
Externally publishedYes


  • Calanthe
  • Conservation
  • Endangered orchid
  • Natural hybridization
  • Next-generation sequencing

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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